Affinity chromatography purification of Clostridium perfringens enterotoxin.
AUTOR(ES)
Scott, V N
RESUMO
Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin purified by Sephadex G-100 chromatography followed by affinity chromatography appears similar to toxin highly purified by conventional means. The procedure can be used successfully for the rapid (less than 2 h) purification of small amounts of enterotoxin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415320Documentos Relacionados
- Affinity Chromatography Purification of Clostridium perfringens Enterotoxin
- Preparative polyacrylamide gel electrophoresis purification of Clostridium perfringens enterotoxin.
- Deletion analysis of the Clostridium perfringens enterotoxin.
- Mapping of functional regions of Clostridium perfringens type A enterotoxin.
- Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.
