Adsorption of the inulinase from Kluyveromyces marxianus NRRL Y-7571 on Streamline® DEAE resin
AUTOR(ES)
Makino, Y., Lima, P. S. C., Filho, F. M., Rodrigues, M. I.
FONTE
Brazilian Journal of Chemical Engineering
DATA DE PUBLICAÇÃO
2005-12
RESUMO
The enzyme inulinase is used to produce oligosaccharides and fructose, with up to 95% fructose in a single stage of inulina hydrolysis. With in the aim to purify the enzyme, studies on the conditions of enzyme adsorption in an expanded-bed column were conducted using phosphate and tris-HCl buffers. The inulinase used in this work was obtained from Kluyveromyces marxianus NRRL Y-7571 by fermentation in an industrial medium. Using the anionic resin Streamline DEAE, the adsorption equilibrium time was determined. It was observed that the adsorption isotherm follows the Langmuir model; the parameters for the maximum amount of adsorbed inulinase (Qm) and the dissociation constant (k d) were determined. With 0.05 M sodium phosphate buffer at pH 6.0, the parameter values 1428 UI/mL and 2 UI/mL with a correlation coefficient of 0.96 were obtained. For 0.02 M tris-HCl buffer at pH 7.5, the same parameters were 5000 UI/mL and 0.05 UI/mL with a correlation coefficient of 0.99. The best purification conditions for the fixed bed were shown to be a 0.4 M phosphate buffer with NaCl as eluter, a purification factor of 11.4, and a recovery yield of up to 79%.
Documentos Relacionados
- Estudo da otimização da produção de inulinase por Kluyveromyces marxianus NRRL Y-7571 em meios industriais pre-tratados.
- Comparative studies of the stability of free and immobilized inulinase from Kluyveromyces marxianus NRRL Y-7571 in aqueous-organic solutions
- Avaliação da produção de fruto-oligossacarídeos a partir de sacarose em meio aquoso e orgânico usando inulinase imobilizada de Kluyveromyces marxianus NRRL Y-7571
- Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract
- Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus