Adequacy of cytogenetic dosimetry to evaluate partial and total body irradiation / AdequaÃÃo da dosimetria citogenÃtica para avaliaÃÃo de irradiaÃÃo parcial e de corpo inteiro

AUTOR(ES)

Thiago de Salazar e Fernandes

DATA DE PUBLICAÇÃO

2009

RESUMO

The majority of human exposures to ionizing radiation involves partial human body irradiation. Thus, the use of cytogenetic dosimetry, when this aspect is not taken into account, can result in dose underestimation to the irradiated subject. In this context, this research studied methodological parameters of biodosimetry, such as cell culture time, time of Colcemid addition and cytogenetic stain methods, with the purpose of providing a planning of choice of the most adequate method considering the type of irradiation (partial or total body). For this, blood samples from a healthy donor were irradiated with doses of 1.5, 3.0, 4.0 Gy of X-rays. The staining methods, Giemsa, DAPI, C banding and FISH were compared in relation to the efficacy in identifying chromosome aberrations, from samples irradiated with 1.5 and 3.0 Gy. Partial body irradiation was simulated by the mixture of 70% blood sample irradiated with 4.0 Gy with 30% non-irradiated sample. The results obtained in this research indicate that the comparison between the frequencies of chromosome aberrations obtained with the time of cell culture of 48 hours, with the ones quantified after prolonged cell culture of 72 hours, allows distinguishing between partial and total body exposure, due to mitotic delay of the population of irradiated lymphocytes. On the other hand, cell cultures with the previous addition of Colcemid and duration of 72 hours, despite of guaranteeing cells in first mitotic division (M1), presented highly condensed chromosomes. In relation to the stain techniques, despite the fact that there are no significant differences between the results of dose estimates among the evaluated techniques, it was observed that FISH and C banding methods are indicated to elucidate doubtable images

ASSUNTO(S)

biodosimetry fish - method biodosimetria x-rays aberraÃÃes cromossÃnicas bandeamento c chromosome aberrations engenharia nuclear energia nuclear cultivo celular fish - mÃtodo cell culture irradiaÃÃes parciais

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