A study on clavulanic acid production by Streptomyces clavuligerus in batch, fed-batch and continuous processes

AUTOR(ES)

Neto, A. B., Hirata, D. B., Cassiano Filho, L. C. M., Bellão, C., Badino Júnior, A. C., Hokka, C. O.

FONTE

Brazilian Journal of Chemical Engineering

DATA DE PUBLICAÇÃO

2005-12

RESUMO

Clavulanic acid (CA) is a potent inhibitor of beta-lactamases, enzymes that are responsible for the hydrolysis of beta-lactam antibiotics. It is a secondary metabolite produced by the filamentous aerobic bacterium Streptomyces clavuligerus in submerged cultivations. In the present work clavulanic acid production in batch, fed-batch and continuous bioreactors was studied with the objective of increasing productivity. The operating conditions: temperature, aeration and agitation, were the same in all cases, 28º C, 0.5 vvm and 800 rpm, respectively. The CA concentration obtained in the fed-batch culture, 404 mg L-1, was ca twice the value obtained in the batch culture, 194 mg L-1, while 293 mg L-1 was obtained in the continuous culture. The highest productivity was obtained in the continuous cultivation, 10.6 mg L-1 h-1, as compared with 8.8 mg L-1 h-1 in the fed-batch process and 3.5 mg L-1 h-1 in the batch process, suggesting that continuous culture of Streptomyces clavuligerus is a promising strategy for clavulanic acid production.

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