A role for exon sequences in alternative splicing of the human fibronectin gene.

AUTOR(ES)

Mardon, H J

RESUMO

Exon EDIIIA of the fibronectin (Fn) gene is alternatively spliced via pathways which either skip or include the whole exon in the messenger RNA (mRNA). We have investigated the role of EDIIIA exon sequences in the human Fn gene in determining alternative splicing of this exon during transient expression of alpha globin/Fn minigene hybrids in HeLa cells. We demonstrate that a DNA sequence of 81bp within the central region of exon EDIIIA is required for alternative splicing during processing of the primary transcript to generate both EDIIIA+ and EDIIIA- mRNA's. Furthermore, alternative splicing of EDIIIA only occurs when this sequence is present in the correct orientation since when it is in antisense orientation splicing always occurs via exon-skipping generating EDIIIA- mRNA.

Documentos Relacionados

• Regulation of Fibronectin EDA Exon Alternative Splicing: Possible Role of RNA Secondary Structure for Enhancer Display
• Exon as well as intron sequences are cis-regulating elements for the mutually exclusive alternative splicing of the beta tropomyosin gene.
• cis-regulatory sequences responsible for alternative splicing of the Drosophila dopa decarboxylase gene.
• Alternative Splicing of the Fibronectin EIIIB Exon Depends on Specific TGCATG Repeats
• A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon.