A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon.
AUTOR(ES)
Tamkun, J W
RESUMO
Three fibronectin mRNAs exist in rat liver, differing by the presence or absence of segments of 285 or 360 bases at a point within the coding region. We previously proposed that the three mRNAs are encoded by a single gene and arise via alternative splicing of a common transcript. In order to test this hypothesis, we have isolated clones spanning approximately half of the fibronectin gene from a Fisher rat genomic library; blot hybridization analyses reveal the presence of only one fibronectin gene in the haploid rat genome. We determined the sequence of a portion (1221 nucleotides) of this gene. This sequence shows clearly that the three fibronectin mRNAs encoded by this gene are generated by a pattern of alternative splicing in which one 5' splice site can be paired with any one of three 3' splice sites, one at the beginning of, and two within, a single complex exon.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391653Documentos Relacionados
- Expression of a human alpha-globin/fibronectin gene hybrid generates two mRNAs by alternative splicing.
- The rat alpha-tropomyosin gene generates a minimum of six different mRNAs coding for striated, smooth, and nonmuscle isoforms by alternative splicing.
- mRNAs for plasma membrane calcium pump isoforms differing in their regulatory domain are generated by alternative splicing that involves two internal donor sites in a single exon.
- Epstein-Barr virus mRNAs produced by alternative splicing.
- Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon.