A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy
AUTOR(ES)
Mor, Natalia Cristina; Correia, Banny S. B.; Val, Adalberto Luis; Tasic, Ljubica
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2020-04
RESUMO
This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods.
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