A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy

This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in di...