Aqueous Twophase Systems
Mostrando 1-12 de 74 artigos, teses e dissertações.
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1. New Insights into the Partitioning of Phenothiazine Dyes in Aqueous Two-Phase Systems
Aqueous two-phase systems (ATPSs) have proven to be efficient and environmentally safe methods for extracting chemical species. However, the driving forces behind the partition of solutes in these systems are still little understood. A complete thermodynamic partitioning of phenothiazine dyes was investigated in poly(ethylene oxide) (PEO) + salt + water ATPS
J. Braz. Chem. Soc.. Publicado em: 2020-08
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2. A Sustainable Methodology to Extract Bismuth from Secondary Sources
Bismuth is a critical metal broadly used in cosmetic, medicine and metallurgy. However, its scarcity in Earth’s crust may impair further applications. An alternative is to recover bismuth from secondary sources. In this work, a methodology to extract bismuth from safety valves of discharged gas cylinders is proposed. Extraction and purification of bismuth
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
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3. Produção e purificação integrada de protease fibrinolítica de Mucor subtilissimus UCP 1262
RESUMO As enzimas fibrinolíticas podem ser obtidas de micro-organismos por meio de processos fermentativos. O presente trabalho teve como objetivo avaliar a produção e extração integrada da protease fibrinolítica de Mucor subtilissimus UCP 1262 usando sistema de duas fases aquosas (SDFA). O processo integrado foi realizado para avaliar a produção, pa
Arq. Bras. Med. Vet. Zootec.. Publicado em: 06/06/2019
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4. Liquid-liquid equilibria in aqueous two-phase ethanol/salt systems at different temperatures and their application to anthocyanins extraction
Abstract Aqueous two-phase systems (ATPS) are formed by mixtures of chemical species that, under certain conditions, separate into two immiscible phases, with water as the major component in global terms and, at lower concentrations, polymers, salts, ionic liquids and alcohols, depending on the system type. Different parameters influence ATPS equilibrium suc
Food Sci. Technol. Publicado em: 16/05/2019
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5. Liquid-Liquid Equilibrium Data, Viscosities, Densities, Conductivities, and Refractive Indexes of Poly (vinyl pyrrolidone)+ tri-sodium Citrate + Urea Aqueous Two-Phase Systems at Different pH
Abstract The present study investigated liquid-liquid equilibria of polyvinylpyrrolidone (PVP) K30 + tri-sodium citrate + urea (5% and 10% mass) aqueous two-phase systems at 25°C and pH values of 6.2, 7.4, and 10.0. Results showed that the binodal was displaced toward higher concentrations as the urea concentration increased. The effect of pH, polymer/salt
Braz. J. Chem. Eng.. Publicado em: 2017-07
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6. Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all
Braz. arch. biol. technol.. Publicado em: 2015-02
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7. Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems
Aqueous two-phase micellar systems (ATPMS) can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, which is influenced by the presence of electrolytes. In this work, we provide an investigation on the cloud point behavior of the nonion
Braz. J. Chem. Eng.. Publicado em: 2014-12
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8. An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up me
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 2012
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9. Produção, caracterização bioquímica e purificação de fitase produzida por Aspergillus niger var. phoenicis URM 4924.
During ripening, vegetable seeds and grain accumulated substantial amount of phytic acid, representing over 60% of total phosphorus in them. Phytate acts as an energy source for seed germination and is rarely available for non-ruminants, since they do not synthesize the enzyme. Due to the unavailability of biological organic phosphorus in many vegetable food
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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10. Sistemas aquosos bifásicos: determinação de equilíbrio de fase e aplicação para partição de alfa-lactoalbumina e beta-lactoglobulina / Aqueous two-phase systems: determination of phase equilibrium and application for partition of alpha-lactoalbumin and beta-lactoglobulin
Este trabalho apresenta, no primeiro capítulo, uma breve revisão sobre obtenção do soro do leite e sua composição, destacando as propriedades nutricionais e tecnológicas das proteínas do soro. Posteriormente, aborda os principais métodos de separação das proteínas do soro, apresentando a utilização de sistemas aquosos bifásicos (SABs) como uma
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/02/2011
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11. Partição da lectina de Canavalia grandiflora Benth em sistemas de duas fases aquosas e aplicação em processo cicatricial / Partition of the lectin from Canavalia grandiflora Benth in aqueous two-phase systems and application healing
The purification process is a limiting step of biomolecules for use in processes in biotechnology, especially lectins, due to the cost of up to 90%, and the large number of steps to obtain the pure molecule, resulting in a low yield. Thus, the aqueous two-phase system (ATPS) was proposed as an alternative for the purification of lectin from Canavalia grandif
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/10/2010
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12. Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold puri
Brazilian Archives of Biology and Technology. Publicado em: 2010-10