Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design
AUTOR(ES)
Antelo, Francine Silva, Costa, Jorge Alberto Vieira, Kalil, Susana Juliano
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2015-02
RESUMO
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w) PEG 4000 or 4% (w/w) PEG 6000 and 21% (w/w) phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase.
Documentos Relacionados
- Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems
- Experimental design as a tool for optimization of C-phycocyanin purification by precipitation from Spirulina platensis
- C-phycocyanin extraction from Spirulina platensis wet biomass
- Kinetics and adsorption isotherm of C-phycocyanin from Spirulina platensis on ion-exchange resins
- Two-phase aqueous micellar systems: an alternative method for protein purification