Aqueous Two Phase Systems
Mostrando 13-24 de 111 artigos, teses e dissertações.
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13. Enfermidades Cutâneas Digitais Bovina: Aspectos Genéticos E Terapêuticos / Digital dermatitis disease bovine: genetic and therapeutic aspects
This study has aimed do evaluate a risk factor for bovine digital dermatitis and the efficacy of 5% aqueous barbatimão extract solution as ancillary in interdigital skin incisional wound repair. One hundred bovine females, 50 Holstein and 50 Girolando, from two different farms were used for the risk factor study. Experimental units were divided into four gr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/03/2011
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14. Produção, caracterização bioquímica e purificação de fitase produzida por Aspergillus niger var. phoenicis URM 4924.
During ripening, vegetable seeds and grain accumulated substantial amount of phytic acid, representing over 60% of total phosphorus in them. Phytate acts as an energy source for seed germination and is rarely available for non-ruminants, since they do not synthesize the enzyme. Due to the unavailability of biological organic phosphorus in many vegetable food
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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15. Sistemas aquosos bifásicos: determinação de equilíbrio de fase e aplicação para partição de alfa-lactoalbumina e beta-lactoglobulina / Aqueous two-phase systems: determination of phase equilibrium and application for partition of alpha-lactoalbumin and beta-lactoglobulin
Este trabalho apresenta, no primeiro capítulo, uma breve revisão sobre obtenção do soro do leite e sua composição, destacando as propriedades nutricionais e tecnológicas das proteínas do soro. Posteriormente, aborda os principais métodos de separação das proteínas do soro, apresentando a utilização de sistemas aquosos bifásicos (SABs) como uma
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/02/2011
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16. Partição da lectina de Canavalia grandiflora Benth em sistemas de duas fases aquosas e aplicação em processo cicatricial / Partition of the lectin from Canavalia grandiflora Benth in aqueous two-phase systems and application healing
The purification process is a limiting step of biomolecules for use in processes in biotechnology, especially lectins, due to the cost of up to 90%, and the large number of steps to obtain the pure molecule, resulting in a low yield. Thus, the aqueous two-phase system (ATPS) was proposed as an alternative for the purification of lectin from Canavalia grandif
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/10/2010
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17. Análise da viabilidade econômica da purificação da bromelina das folhas de curauá em sistema bifásico aquoso PEG/Fosfato / Economic viability of bromelain purification from curaua using an aqueous two phase system PEG/phosphate
O curauá (Ananas Erectifolius L.B. Smith), espécie vegetal de porte herbáceo, é uma monocotiledônea pertencente à família Bromeliaceae, nativa da região norte do Brasil, especialmente da Amazônia, de onde pode ser extraída a bromelina. Essa faz parte de um grupo de enzimas proteolíticas, usadas na indústria alimentícia e como medicamento, pois o
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/06/2010
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18. Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold puri
Brazilian Archives of Biology and Technology. Publicado em: 2010-10
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19. Alternativas verdes para o preparo de amostra e determinação de poluentes fenólicos em água
The restricted availability of water sources suitable for consumption and high costs for obtaining potable water has caused an increase of the conscience concerning the use. Thus, there is a high demand for "environmentally safe methods" which are according to the principles of Green Chemistry. Moreover, these methods should be able to provide reliable resul
Química Nova. Publicado em: 2010
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20. Production and extraction of clavulanic acid from Streptomyces spp. by extractive fermentation using aqueous two-phase system / Produção e extração de ácido clavulânico de Streptomyces spp. por fermentação extrativa utilizando sistemas de duas fases aquosas
Clavulanic acid (CA) is a potent inhibitor of β-lactamases used in the medical field. Alternative methods, economic and simple purification are of great interest. This PhD project aims to produce and extract clavulanic acid of Streptomyces spp. By extractive fermentation using aqueous two-phase system (ATPS) - Polyethylene glycol (PEG)/phosphate salts.
Publicado em: 2010
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21. Extraction and purification of C-phycocyanin from Spirulina platensis in conventional and integrated aqueous two-phase systems
Extrato aquoso de C-ficocianina de Spirulina platensis, com e sem resíduos celulares, foi purificado em sistema aquoso bifásico (SAB) polietilenoglicol (PEG)/fosfato de potássio variando-se a massa molar do PEG, a pH 6, mais promissor do que a pH 7. Para os ensaios realizados com cada massa molar, com diferentes percentuais de PEG e sal, as concentraçõe
Journal of the Brazilian Chemical Society. Publicado em: 2010
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22. Equilibrio de fases de sistemas contendo polimeros sinteticos e carboidratos em meio aquoso / Phase equilibrium of systems containing synthetic polymers and carbohydrates in aqueous medium
This work relates phase equilibrium of binary and ternary systems formed by aqueous mixtures of EO-PO block co-polymers of five different molar masses and EO/PO ratios, and maltodextrins of three different mean molar masses studied at 298.15 K. 25 Liquid-Liquid Equilibrium (LLE) phase diagrams for ternary systems at temperatures ranging from 288,15 to 308,15
Publicado em: 2009
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23. Um método verde e sensível para determinação de fenóis em amostras de água utilizando sistemas aquosos bifásicos / A greener and sensitive method for phenols determination in water samples using aqueous twophase systems
This work initially presents a brief review of aqueous two- phase systems (ATPS), including the history, principles and properties of the technique. Furthermore, a divulgation on green methods for determination of phenols in water is shown, since these substances have high toxicity. The disposal of phenolic compounds is regulated in several countries, includ
Publicado em: 2009
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24. Equilíbrio líquido-líquido de sistemas aquosos constituídos por copolímero tribloco e sal em diferentes temperaturas / Liquid-liquid equilibrium of aqueous systems consisting of triblock copolymer and salt at different temperatures
Phases diagrams of aqueous biphasic systems (ABS) formed by triblock copolymer+salt+H2O were construed at three temperatures: The ABS L64+Na2SO4+H2O and L64+Li2SO4+H2O at 5, 10 and 25 C and ABS L35+Na2CO3+H2O, F68+Na3C6H5O7+H2O, F68+Na2C4H4O6+H2O, F68+Na2CO3+H2O and F68+Na2C4H4O4+H2O at 10, 25 and 40 C. The effect of temperature on the position of the binoda
Publicado em: 2009