Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
AUTOR(ES)
Karthikayalu, S, Rama, V, Venkatesan, R
FONTE
Journal of Venomous Animals and Toxins including Tropical Diseases
DATA DE PUBLICAÇÃO
2010
RESUMO
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis against 10 mM sodium phosphate buffer (pH 7.4), followed by anion exchange chromatography in DEAE-Sepharose® column (GE Healthcare, Sweden) and gel filtration chromatography using Sephadex® G-50 matrix (GE Healthcare, Sweden). The active fractions from the gel filtration chromatography were pooled and rechromatographed in the same column. The final active fraction showed a prominent protein band of molecular mass of 19 kDa when analyzed by SDS-PAGE.
Documentos Relacionados
- Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean
- Crystallization and preliminary crystallographic analysis of fragaceatoxin C, a pore-forming toxin from the sea anemone Actinia fragacea
- Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
- Outer membrane protein K of Escherichia coli: purification and pore-forming properties in lipid bilayer membranes.
- Pore-forming peptide of pathogenic Entamoeba histolytica.