Aminotriazole
Mostrando 25-36 de 49 artigos, teses e dissertações.
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25. Nonphotosynthetic Retardation of Chloroplast Senescence by Light 1
Excised apical portions of green wheat leaf sections were treated with aminotriazole to prevent formation of new chloroplasts. Illumination retarded the decline in chlorophyll content per leaf section, the disintegration of chloroplast ultrastructure, and the loss of capacity for photosynthetic carbon fixation. We interpret these 3 effects of illumination as
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26. Ultrastructural Localization of Hydrogen Peroxide Production in Ligninolytic Phanerochaete chrysosporium Cells †
Previous studies have shown that the hydroxyl radical derived from hydrogen peroxide (H2O2) is involved in lignin degradation by Phanerochaete chrysosporium. In the present study, the ultrastructural sites of H2O2 production in ligninolytic cells of P. chrysosporium were demonstrated by cytochemically staining cells with 3,3′-diaminobenzidine (DAB). Hydrog
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27. Crl Mutants of Saccharomyces Cerevisiae Resemble Both Mutants Affecting General Control of Amino Acid Biosynthesis and Omnipotent Translational Suppressor Mutants
Cyocloheximide resistant lethal (crl) mutants of Saccharomyces cerevisiae, defining 22 unlinked complementation groups, are unable to grow at 37°. They are also highly pleiotropic at their permissive temperature of 25°. The mutants are all unable to arrest at the G(1) stage of the cell cycle when grown to stationary phase or when starved for a single amino
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28. Translocation of Sulfate in Soybean (Glycine max L. Merr) 1
Sulfate translocation in soybean (Glycine max L. Merr) was investigated. More than 90% of the sulfate entering the shoot system was recoverable in one or two developing trifoliate leaves. In young plants, the first trifoliate leaf contained between 10 to 20 times as much sulfate as the primary leaves, even though both types of leaf had similar rates of trans
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29. Uptake and metabolism of iodine is crucial for the development of thyroiditis in obese strain chickens.
To assess the importance of the role of thyroidal iodine in the pathogenesis of thyroiditis in the obese strain (OS) chicken, a model of spontaneous and severe disease, we studied the effect of antithyroid drugs that reduce thyroidal iodine or prevent its metabolism. Reduction of thyroidal iodine was achieved with KClO4, an inhibitor of iodine transport and
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30. The Pattern of Systemic Acquired Resistance Induction within the Arabidopsis Rosette in Relation to the Pattern of Translocation1
Local leaf infections by a necrogenic pathogen can lead to systemic acquired resistance (SAR) in untreated leaves. We reasoned that, whatever the nature of the long-distance signal, if it is transported in the phloem, the pattern of SAR induced within the plant by treatment of a single leaf should match the pattern of translocation out of that leaf. The
The American Society for Plant Biologists.
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31. Detection of a novel catalase in extracts of Mycobacterium avium and Mycobacterium intracellulare.
A novel class of catalase, which differs from the previously described M- and T-catalases of mycobacteria, was detected in strains of Mycobacterium avium and M. intracellulare. Designated A-catalase, this enzyme resisted inactivation at 68 degrees C, was inactivated by 3-amino-1,2,4-triazole (aminotriazole), and exhibited no peroxidase activity. All of these
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32. Oxidative tyrosylation of high density lipoprotein by peroxidase enhances cholesterol removal from cultured fibroblasts and macrophage foam cells.
Lipoprotein oxidation is thought to play a pivotal role in atherogenesis, yet the underlying reaction mechanisms remain poorly understood. We have explored the possibility that high density lipoprotein (HDL) might be oxidized by peroxidase-generated tyrosyl radical. Exposure of HDL to L-tyrosine, H2O2, and horseradish peroxidase crosslinked its apolipoprotei
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33. Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.
Saccharomyces cerevisiae contains eight members of a novel and fungus-specific family of bZIP proteins that is defined by four atypical residues on the DNA-binding surface. Two of these proteins, Yap1 and Yap2, are transcriptional activators involved in pleiotropic drug resistance. Although initially described as AP-1 factors, at least four Yap proteins bind
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34. Mechanisms of methylene blue stimulation of the hexose monophosphate shunt in erythrocytes.
The response of the hexose monophosphate shunt in erythrocytes was studied with the ionization chamber-electrometer apparatus to measure continuously 14CO2 derived from 14C-labeled substrates. The effect of methylene blue at high (0.1 mM) and low (1 muM) concentrations was evaluated under different gas mixtures; air, carbon monoxide, and 6% carbon monoxide i
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35. Tyrosyl radical generated by myeloperoxidase catalyzes the oxidative cross-linking of proteins.
Phagocytes generate H2O2 for use by a secreted heme enzyme, myeloperoxidase, to kill invading bacteria, viruses, and fungi. We have explored the possibility that myeloperoxidase might also convert L-tyrosine to a radical catalyst that cross-links proteins. Protein-bound tyrosyl residues exposed to myeloperoxidase, H2O2, and L-tyrosine were oxidized to o,o'-d
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36. Evidence for Chilling-Induced Oxidative Stress in Maize Seedlings and a Regulatory Role for Hydrogen Peroxide.
We have taken advantage of an acclimation phenomenon in a chilling-sensitive maize inbred to investigate the molecular, biochemical, and physiological responses to chilling in preemergent maize seedlings. Three-day-old seedlings were exposed to 4[deg]C for 7 days and did not survive chilling stress unless they were preexposed to 14[deg]C for 3 days. cDNAs re