Aminotriazole
Mostrando 1-12 de 49 artigos, teses e dissertações.
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1. Identificação de um fator do hospedeiro, RPS5A, envolvido na interação com a proteína de movimento (MP) de geminivírus / Identification of movement protein MP-intaracting host facotrs
The movement protein MP from bipartite geminivirus (begomovirus) facilitates the cell-to-cell and long-distance transport of viral DNA in addition to affecting viral pathogenicity. To identify host factors that interact with MP, initially a cDNA library prepared from CaLCuV (Cabbage leaf curl virus)-infected Arabidopsis leaf mRNA was generated in a pEXPAD502
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/02/2011
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2. SYNCHRONY BETWEEN CATALASES AND ASCORBATE PEROXIDASES IN COWPEA LEAVES PROTECTION AGAINST THE WATER AND SALT STRESS-INDUCED OXIDATIVE DAMAGE / Sincronia entre catalases e peroxidases de ascorbato na proteÃÃo contra danos oxidativos em folhas de feijÃo Caupi expostas aos estresses hÃdrico e salino
O estresse hÃdrico induzido por seca ou salinidade à a principal restriÃÃo ambiental à sobrevivÃncia das plantas e sustentabilidade das culturas, principalmente nas regiÃes semiÃridas, onde estÃo frequentemente associados a altas temperaturas e taxas de luminosidade. Uma grande parte dos efeitos destes estresses ambientais no metabolismo das plantas
Publicado em: 2007
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3. ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance.
In Saccharomyces cerevisiae, 3-amino-1,2,4-triazole (aminotriazole) competitively inhibits the activity of imidazoleglycerolphosphate dehydratase, the product of the HIS3 gene. Wild-type strains are able to grow in the presence of 10 mM aminotriazole because they induce the level of imidazoleglycerolphosphate dehydratase. However, strains containing gcn4 mut
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4. Catalase-Aminotriazole Assay, an Invalid Method for Measurement of Hydrogen Peroxide Production by Wood Decay Fungi
The catalase-aminotriazole assay for determination of hydrogen peroxide apparently cannot be used for measuring hydrogen peroxide production in crude preparations from wood decay fungi because of materials in the crude preparations that interfere with the test.
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5. Formation of diiodotyrosine from thyroxine. Ether-link cleavage, an alternate pathway of thyroxine metabolism.
Studies were performed to elucidate the nature of the pathway of hepatic thyroxine (T4) metabolism that is activated by inhibitors of liver catalase. For this purpose, the metabolism of T4 in homogenates of rat liver was monitored with T4 labeled with 125I either at the 5'-position of the outer-ring (125I-beta-T4) or uniformly in both the outer and inner rin
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6. Glucose-dependent Secretion and Destruction of Hydrogen Peroxide by Mycoplasma pneumoniae
The secretion of H2O2 by Mycoplasma pneumoniae and M. gallisepticum was measured with the new catalase-aminotriazole method. Peroxide secretion by the mycoplasmas was stimulated by glucose. When catalase and aminotriazole were omitted and exogenous H2O2 was added to the mycoplasmas, a loss in H2O2 was noted with time; the addition of glucose speeded the disa
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7. Oxidative Stimulation of Glutathione Synthesis in Arabidopsis thaliana Suspension Cultures.
A system based on Arabidopsis thaliana suspension cultures was established for the analysis of glutathione (GSH) synthesis in the presence of hydrogen peroxide. Mild oxidative stress was induced by use of the catalase inhibitor, aminotriazole, and its development was monitored by measurement of the oxidative inactivation of aconitase. Addition of 2 mM aminot
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8. Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical.
The ability of intact human red cells to scavenge extracellularly generated H2O2 and O2-, and to prevent formation of hydroxyl radicals and hypochlorous acid has been examined. Red cells inhibited oxidation of ferrocytochrome c by H2O2. Cells treated with aminotriazole no longer inhibited, indicating that protection was almost entirely due to intracellular c
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9. Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis
Evidence for the presence of peroxidative metabolism in rabbit alveolar macrophages (AM) has been obtained from the following observations: (a) catalase is present in high concentrations; (b) peroxidase activity could not be detected employing guaiacol as substrate; (c) the irreversible inhibition of AM catalase by aminotriazole served as a detection system
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10. Hydrogen Peroxide Metabolism in Soybean Embryonic Axes at the Onset of Germination 1
Hydrogen peroxide steady state levels of 5 micromolar were determined in soybean (Glycine max) embryonic axes incubated for 2 hours and in axes pretreated with aminotriazole or cyanide, where these levels were 50 and 1 micromolar, respectively. The activities of catalase (105 picomoles H2O2 per minute per axis), peroxidase (10-44 picomoles H2O2 per minute pe
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11. Mutations causing aminotriazole resistance and temperature sensitivity reside in gyrB, which encodes the B subunit of DNA gyrase.
Certain mutations in gyrA and gyrB, the genes encoding the two subunits of DNA gyrase, are known to influence expression of the his operon (K. E. Rudd and R. Menzel, Proc. Natl. Acad. Sci. USA 84:517-521, 1987). Such mutations lead to a decrease in tRNA(His) levels and consequently to an attenuator-dependent increase in his operon expression. This effect pre
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12. Control of Demand-Driven Biosynthesis of Glutathione in Green Arabidopsis Suspension Culture Cells1
We have investigated what limits demand-driven de novo glutathione (GSH) biosynthesis in green Arabidopsis suspension culture cells. GSH is the most abundant low-molecular weight thiol in most plants and can be quantified using monochlorobimane to fluorescently label GSH in live cells. Progress curves for labeling reached a plateau as all the cytoplasmic GSH
American Society of Plant Biologists.