Alizarin Red Staining
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells
Abstract: The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expressi
Braz. oral res.. Publicado em: 10/01/2020
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2. Evaluation of dentinogenesis inducer biomaterials: an in vivo study
Abstract When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect
J. Appl. Oral Sci.. Publicado em: 28/11/2019
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3. Low-intensity pulsed ultrasound promotes tissue regeneration in rat dental follicle cells in a porous ceramic scaffold
Abstract The aim of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the osteogenic differentiation of dental follicle cells (DFCs) in vitro and on the regenerative effects of DFC-OsteoBoneTM complexes in vivo. DFCs were isolated and characterized. In the in vitro study, DFCs were cultured in an osteogenic medium in the
Braz. oral res.. Publicado em: 09/09/2019
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4. Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells
Abstract Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. Objective To investigate the effect of calcium hyd
J. Appl. Oral Sci.. Publicado em: 11/04/2019
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5. Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro
Abstract Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and ge
J. Appl. Oral Sci.. Publicado em: 08/11/2018
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6. Physical properties and biological effects of mineral trioxide aggregate mixed with methylcellulose and calcium chloride
Abstract Objectives: Methylcellulose (MC) is a chemical compound derived from cellulose. MTA mixed with MC reduces setting time and increases plasticity. This study assessed the influence of MC as an anti-washout ingredient and CaCl2 as a setting time accelerator on the physical and biological properties of MTA. Material and Methods: Test materials were di
J. Appl. Oral Sci.. Publicado em: 2017-12
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7. Human plasma fibronectin promotes proliferation and differentiation of odontoblast
Abstract Objective To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro. Material and Methods Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkaline phosphatase activity (ALP activity), mRNA expressio
J. Appl. Oral Sci.. Publicado em: 2017-06
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8. Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated wi
Braz J Med Biol Res. Publicado em: 11/07/2016
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9. Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHC
Braz. arch. biol. technol.. Publicado em: 27/06/2016
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10. Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis
ABSTRACT Objective Biocompatible materials such as interconnected porous hydroxyapatite ceramics (IP-CHA) loaded with osteogenic cells and bioactive agents are part of an evolving concept for overcoming craniofacial defects by use of artificial bone tissue regeneration. Amongst the bioactive agents, melatonin (MEL) and basic fibroblast growth factor (FGF-2
J. Appl. Oral Sci.. Publicado em: 2016-04
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11. Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
ABSTRACT Objective The purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. Material and Method To evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measur
J. Appl. Oral Sci.. Publicado em: 2016-02
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12. Bioactivity, physical and chemical properties of MTA mixed with propylene glycol
AbstractObjective To investigate the physical (setting time, hardness, flowability, microstructure) and chemical (pH change, calcium release, crystallinity) properties and the biological outcomes (cell survival and differentiation) of mineral trioxide aggregate (MTA) mixed using different proportions of propylene glycol (PG) and water.Material and Methods Wh
J. Appl. Oral Sci.. Publicado em: 2015-08