Alizarin Red Staining
Mostrando 13-24 de 24 artigos, teses e dissertações.
-
13. Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells
Primary human bone marrow stromal cells (hMSCs) were transfected with human telomerase reverse transcriptase (hTERT) gene with lipofection method. The hTERT transfected hMSCs of passage 100 underwent chondrogenesis induction with dexamethasone, transforming the growth factor β and vitamin C, osteogenesis induction with dexamethasone, β glycerophosphoric ac
Braz. arch. biol. technol.. Publicado em: 2014-02
-
14. In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated
Acta Cir. Bras.. Publicado em: 2013-05
-
15. In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biologic
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-06
-
16. Efeitos da fototerapia com laser em baixa intensidade e dos fatores de crescimento PDGF e BMP-2, isolados ou em associação, na diferenciação ósseo/odontogênica de células-tronco de polpa dentária humana / Effects of low intensity laser therapy and growth factors PDGF and BMP-2 on the odontogenic differentiation of dental pulp stem cells
A fototerapia com laser em baixa intensidade (FTLBI) é capaz de aumentar o metabolismo celular, o que poderia influenciar na diferenciação ósseo/odontogênica das células-tronco da polpa dentária humada (hDPSCs). O PDGF e o BMP-2 são fatores de crescimento envolvidos na dentinogênese e na reparação tecidual. O PDGF tem papel importante durante o de
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/09/2011
-
17. Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis
OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in t
Clinics. Publicado em: 2008
-
18. Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction.
Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain alizarin red S provides a simple, quick technique for visualisation of both damaged and normal cells, thereby permitting the quantification of endothelial cell damage. Adjustment of the pH of the alizarin red S reagent to 4.2 is impor
-
19. Alendronate Enhances Osteogenic Differentiation of Bone Marrow Stromal Cells: A Preliminary Study
Alendronate inhibits osteoclastic activity. However, some studies suggest alendronate also has effects on osteoblast activity. We hypothesized alendronate would enhance osteoblastic differentiation without causing cytotoxicity of the osteoblasts. We evaluated the effect of alendronate on the osteogenic differentiation of mouse mesenchymal stem cells. D1 cell
Springer-Verlag.
-
20. The in vivo staining of bone with alizarin red S.
-
21. Pathogenesis of corneal oedema associated with herpetic eye disease.
Corneal oedema and stromal disease, induced in rabbits by intrastromal injection of herpes simplex virus, type 1, strain RE (HSV-1, RE), reached a peak of 12-15 days after infection. Corneal oedema as measured by ultrasonic pachymetry, and stromal disease as measured by a subjective scoring system, were closely related for 30 days after infection. Morphometr
-
22. Visualization of Proteus mirabilis within the Matrix of Urease-Induced Bladder Stones during Experimental Urinary Tract Infection
The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mu
American Society for Microbiology.
-
23. Migration of Calcium and Its Role in the Regulation of Seismonasty in the Motor Cell of Mimosa pudica L. 1
Volume and conformational changes of the contractile tannin vacuoles of the abaxial motor cells of the primary pulvinus of Mimosa pudica L. parallel the seismonastic leaf movement. Since such changes in cells and organelles of animal systems are often regulated by calcium, we studied Ca2+ movement in the motor cells and tissue. By fixation with Lillie's neut
-
24. Osteogenic Cells Derived From Embryonic Stem Cells Produced Bone Nodules in Three-Dimensional Scaffolds
An approach for 3D bone tissue generation from embryonic stem (ES) cells was investigated. The ES cells were induced to differentiate into osteogenic precursors, capable of proliferating and subsequently differentiating into bone-forming cells. The differentiated cells and the seeded scaffolds were characterized using von Kossa and Alizarin Red staining, ele
Hindawi Publishing Corporation.