Agrobacterium Tumefaciens
Mostrando 1-12 de 999 artigos, teses e dissertações.
-
1. Genotyping and molecular analysis of transgenic sequences in chrysanthemum (Chrysanthemum X morifolium Ramat)
Abstract Genotypes of the chrysanthemum cultivars Harman and Indianapolis were transformed using Agrobacterium tumefaciens strain LBA4404, which carries the binary plasmid pBIN19::Rd29A::ScTPS1TPS2::nos and pBIN19::35S::ScTPS1TPS2::nos, respectively. ISSR markers were used to discriminate two cultivars from each other, and independent transgenic plants gener
Crop Breed. Appl. Biotechnol.. Publicado em: 31/10/2019
-
2. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resist
Genet. Mol. Biol.. Publicado em: 13/06/2019
-
3. Molecular farming of antimicrobial peptides: available platforms and strategies for improving protein biosynthesis using modified virus vectors
Abstract The constant demand for new antibiotic drugs has driven efforts by the scientific community to prospect for peptides with a broad spectrum of action. In this context, antimicrobial peptides (AMPs) have acquired great scientific importance in recent years due to their ability to possess antimicrobial and immunomodulatory activity. In the last two dec
An. Acad. Bras. Ciênc.. Publicado em: 18/10/2018
-
4. Aumento da transformação genética transiente em embriões imaturos do genótipo brasileiro de milho BR 451 co-cultivados com Agrobacterium tumefaciens
RESUMO. A engenharia genética ampliou as possibilidades do melhoramento genético vegetal, apoiando ideais de sustentabilidade. A Agrobacterium tumefaciens é o sistema de transformação preferido, uma vez que permite a produção de plantas transgênicas com maior estabilidade na expressão gênica e na herdabilidade. A Agrobactéria e o tecido vegetal de
Acta Sci., Agron.. Publicado em: 16/07/2018
-
5. OTIMIZAÇÃO DE FATORES QUE AFETAM A TRANSFORMAÇÃO GENÉTICA DE Eucalyptus saligna MEDIADA POR Agrobacterium tumefaciens
RESUMO Esse trabalho teve como objetivo avaliar o efeito de vários fatores que podem afetar a transformação genética de explantes cotiledonares de Eucalyptus saligna mediante co-cultura com a cepa EHA105 de Agrobacterium tumefaciens. Foi utilizado o vector pBI121 carregando o gene repórter gus sob controle do promotor 35SCaMV. Foi estudado o efeito dos
Rev. Árvore. Publicado em: 22/02/2018
-
6. Beneficial effects of bio-controlling agent Bacillus cereus IB311 on the agricultural crop production and its biomass optimization through response surface methodology
ABSTRACT Disease in agricultural field is a big problem that causes a massive loss in production. In this present investigation, we have reported a soil-borne bacterium Bacillus cereus IB311 which is antagonistic to plant pathogens (Pseudomonas syringae and Agrobacterium tumefaciens), and could make a substantial contribution to the prevention of plant disea
An. Acad. Bras. Ciênc.. Publicado em: 16/10/2017
-
7. Transformação genética da variedade brasileira de milho BR 451 pelo método da Agrobacterium tumefaciens
RESUMO: A introdução de genes de forma assexual por meio da engenharia genética tem ampliado as possibilidades do melhoramento genético vegetal. No entanto, devido principalmente a baixa resposta in vitro, a transformação genética tem se limitado a poucos genótipos das espécies de interesse agronômico. Visando estabelecer protocolo de transformaç
Cienc. Rural. Publicado em: 09/10/2017
-
8. Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea
Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-09
-
9. Isolation and characterization of a high salt-tolerant and glyphosate-degrading strain of Agrobacterium tumefaciens BZ8
ABSTRACT In this study, a high salt-tolerant and glyphosate-degrading strain named BZ8 was isolated from activated sludge. According to 16S rDNA sequencing methods, morphological, physiological and biochemical analysis, strain BZ8 was identified as Agrobacterium tumefaciens. The growth and glyphosate-degrading capability of A. tumefaciens BZ8 were investigat
Braz. J. Chem. Eng.. Publicado em: 2017-07
-
10. Agrobacterium-mediated genetic transformation of a tropical elite maize line
Abstract The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% - half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maiz
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-06
-
11. Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of thes
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09
-
12. Agrotransformação de Phytophthora nicotianae: um método simplificado e otimizado
RESUMO Phytophthora nicotianae é um fitopatógeno responsável por danos em culturas e ecossistemas naturais em todo o mundo. P. nicotianae está relacionada às doencas: gomose e podridao de raízes e o manejo destas doencas depende principalmente de certificação de mudas e erradicação de plantas infectadas. No entanto, pouco se sabe sobre a estratégi
Summa phytopathol.. Publicado em: 2016-09