2 Mercaptoethanol
Mostrando 1-12 de 480 artigos, teses e dissertações.
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1. Occurrence of anti-Brucella abortus agglutinins in small ruminants in Sergipe
RESUMO: Objetivou-se avaliar a ocorrência de pequenos ruminantes soropositivos para Brucella abortus em Sergipe, Brasil, a partir de 1.200 amostras de soro sanguíneo de ovinos e 675 de caprinos, oriundos de 101 propriedades de 25 municípios das três mesorregiões do estado, nas quais foi empregada a técnica do Antígeno Acidificado Tamponado (AAT) corad
Arq. Inst. Biol.. Publicado em: 25/11/2019
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2. Analysis of the risk factors for bovine brucellosis in dairy herds of the Rio Branco microregion, Acre, Brazil
RESUMO A brucelose bovina (BB) é uma bacteriose de notificação obrigatória, causada pela Brucella abortus, responsável por distúrbios reprodutivos graves em bovinos, prejuízos consideráveis ao comércio e agravos em saúde pública. Objetivaram-se com este estudo identificar a frequência de BB e analisar os fatores de risco nos rebanhos leiteiros da
Arq. Inst. Biol.. Publicado em: 10/10/2019
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3. Bovine brucellosis in slaughterhouses controlled by Federal and Municipal Inspection Services in the state of Maranhão, Brazil
RESUMO: Os objetivos do estudo foram determinar a prevalência da brucelose bovina, relacionar resultados laboratoriais de amostras séricas reagentes com a sorologia de animais que apresentavam bursites e, ainda, identificar possíveis fatores de risco para a enfermidade. Para isso, no abate foram coletadas 1.265 amostras de sangue bovino, entre machos e f�
Arq. Inst. Biol.. Publicado em: 30/05/2019
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4. Development and standardization of a western blotting test for detection of antibodies against B. abortus
RESUMO A brucelose é uma doença infectocontagiosa, causada por bactérias do gênero Brucella spp., com diagnóstico baseado no emprego de técnicas sorológicas. Objetivou-se neste estudo desenvolver e padronizar um teste Western blotting (WB) para detecção de anticorpos contra B. abortus. Foram analisados dois grupos de amostras bovinas: grupo I, com 6
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2019-02
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5. Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
Abstract The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood se
Braz. J. Microbiol.. Publicado em: 2018-09
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6. Composite Films from Polystyrene with Hydroxyl end Groups and Carbon Nanotubes
Composites from polystyrene with hydroxyl end groups and multiwall carbon nanotubes were fabricated to evaluate their properties. The Carbon Nanotubes (CNTs) were synthetized by chemical vapor deposition technique using ferrocene and benzene as precursors. Polystyrene with hydroxyl end groups was prepared by solution polymerization employing styrene as monom
Mat. Res.. Publicado em: 09/02/2017
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7. Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10
Abstract Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30 °C for 96 h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipa
Braz. J. Microbiol.. Publicado em: 2016-03
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8. Zoonoses in humans from small rural properties in Jataizinho, Parana, Brazil
The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural
Braz. J. Microbiol.. Publicado em: 05/04/2013
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9. Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins
The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the existence of published results for the optimization of proteomic
Genetics and Molecular Biology. Publicado em: 2012
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10. Soroprevalência de anticorpos anti-Toxoplasma gondii em animais domésticos e silvestres invasores do arquipélago de Fernando de Noronha, PE.
The Archipelago of Fernando de Noronha is known to possess a rich biological diversity, however, as in other islands of Brazil suffered various human activities. Among these actions, there is the introduction of invasive alien species, which can be sources of infection or reservoirs of zoonotic pathogens. The objective of this research was to determine the s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/02/2011
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11. Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity
The properties and agglutination activity of acutolysin C, a hemorrhagic metalloproteinase obtained from Agkistrodon acutus venom, were studied herein. Acutolysin C is a basic glycoprotein consisting of a single polypeptide chain with a molecular weight of 23.1 kDa and pI 8.7, containing one Zn2+ and one Ca²+ per molecule. It possesses caseinolytic, weak le
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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12. Quantificação e pureza do DNA de videira por meio de espectrofotometria.
Neste trabalho, são apresentados valores estimados da quantificação de DNA por espectrofotometria de 219 cultivares procedentes da coleção de Germoplasma de Videira da Embrapa Semi-Árido, localizada no Campo Experimental de Mandacaru, em Juazeiro-BA. O DNA genômico total foi isolado de folhas verdes e sadias, segundo protocolo 500 mM Tris pH 8,0, 1,4
JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO. Publicado em: 2011