Human adenovírus serotype 41 interaction with hematopoietic cells: cellular permissiviness and viral gene expression analysis. / Interação do adenovírus humano, sorotipo 41, com células origem hematopoiética: análise da permissividade celular e da expressão gênica viral.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

In order to verify the permissiveness of hematopoietic cells to HAdV-41 infection, PBMC and IEL from volunteers were infected. The infection assays were compared with infected HEK -293 cells. We analysed the E1A, E1B (55K), E3 (14K), VARNA, hexon and short fiber (SF) viral gene expression and GAPDH cellular gene expression. The mRNA were detected by real time PCR and the viral protein synthesis were detected by IIF. In HEK-293 cells E1A, E1B and E3 gene expression were detected 11h p.i Hexon gene expression was detected at 13h p.i, while VARNA and SF were detected 14h p.i In PBMC, E1A, E1B and VARNA gene expression were detected 17h p.i and the hexon and SF were detected 18h p.i and 20h p.i, respectively. The viral gene expression level in infected HEK-293 cells was 200 fold higher than infected PBMC. The IEL also were permissive to HAdV-41 infection showed by viral gene expressions. This is the first evidence that HAdV-41 is able to infect these cells types. These results helps to understand the virus-cell interaction mechanisms.

ASSUNTO(S)

pbmc adenoviruses adenovírus intraepithelial lymphocytes infection kinetics cellular permissiveness linfócitos intraepiteliais permissividade celular pbmc cinética de infecção

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