Caracterização funcional e estrutural de uma L-Aminoácido oxidace do veneno de Bothrops jararacussue avaliação da sua ação antitumoral, antiparasitária e bactericida / Functional and structural characterization of an L-aminoacid oxidace from Bothrops jararacussuvenom and the evaluation of its antitumoral, antiparasitic and bactericidal action

AUTOR(ES)

Fabio Kiss Ticli

DATA DE PUBLICAÇÃO

2006

RESUMO

In this research we described the isolation and biochemical, structural and functional characterization of an L-amino acid oxidase from Bothrops jararacussu venom, named BjussuLAAO. The purification was carried out through three chromatography steps, using a molecular filtration on Sephadex G-75, followed by an affinity chromatography on Benzamidine-Sepharose, and finally a hydrophobic chromatography on Phenyl-Sepharose. BjussuLAAO is a protein with a molecular mass of 61 kDa and pI 5.8. This protein showed high sequencial similarity with other LAAOs from snake venoms. BjussuLAAO induced edema only in the highest dose (100 ?g/animal), did not show any myotoxic or hemorrhagic activity. BjussuLAAO, showed clotting and fibrinogenolitic activity, even in the presence of metalloproteases and serino-proteases inhibitors. The more interesting pharmacological activities, where from we can look a future application, were the anti-tumoral, bactericide, leishmanicidal and tripanocidal actions. The enzyme displayed a bactericide effect too, showing to be a multifunctional drug.

ASSUNTO(S)

antiparasitária l-aminoacid oxidase bothrops jararacussu l-aminoácido oxidase antiparasitic bactericida antitumoral bothrops jararacussu bactericidal antitumoral

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