Zymography
Mostrando 1-12 de 99 artigos, teses e dissertações.
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1. Alternagin-C binding to α2β1 integrin controls matrix metalloprotease-9 and matrix metalloprotease-2 in breast tumor cells and endothelial cells
Abstract Background Matrix metalloproteinases (MMPs) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. The role of integrins, adhesion receptors that connect cells to the extracellular matrix, in MMP expression and activity has been previously suggested. However, the m
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 24/05/2018
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2. In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai
Abstract Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a seri
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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3. Resistance training and hormone replacement increase MMP-2 activity, quality and quantity of bone in ovariectomized rats
Abstract AIMS The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. METHODS Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): sham-operated sedentary (SHAM Sed),
Motriz: rev. educ. fis.. Publicado em: 21/12/2017
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4. Degradation of monoaromatics by Bacillus pumilus MVSV3
ABSTRACT Monoaromatics, such as benzene, toluene, ethylbenzene and xylene (BTEX), are simple aromatic compound that are highly toxic due to their high solubility nature. Many chemical and physical methods for their degradation and breakup into nontoxic products are available, but still use of microorganism is preferred over these processes. In this present s
Braz. arch. biol. technol.. Publicado em: 22/06/2017
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5. Bee venom induces apoptosis and suppresses matrix metaloprotease-2 expression in human glioblastoma cells
Abstract Glioblastoma is the most common malignant brain tumor representing with poor prognosis, therapy resistance and high metastasis rate. Increased expression and activity of matrix metalloproteinase-2, a member of matrix metalloproteinase family proteins, has been reported in many cancers including glioblastoma. Inhibition of matrix metalloproteinase-2
Rev. bras. farmacogn.. Publicado em: 2017-06
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6. Enhanced production and organic solvent stability of a protease fromBrevibacillus laterosporus strain PAP04
A bacterial strain (PAP04) isolated from cattle farm soil was shown to produce an extracellular, solvent-stable protease. Sequence analysis using 16S rRNA showed that this strain was highly homologous (99%) to Brevibacillus laterosporus. Growth conditions that optimize protease production in this strain were determined as maltose (carbon source), skim milk (
Braz J Med Biol Res. Publicado em: 18/03/2016
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7. Partial Purification and Characterization of β-glucosidase fromMonascus sanguineus
The aim of the present work was to study the production and characterization of β-glucosidase from Monascus sanguineus. Agro-waste residues were screened to obtain the maximum yield of enzyme. Jack fruit seed was the best substrate for enzyme production. Studies on the optimization of pH and temperature showed acidic pH favorable for enzymatic activity, whe
Braz. arch. biol. technol.. Publicado em: 14/10/2014
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8. Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues
The aim of this work was to study the production of fibrinolytic protease by Bacillus subtilis I-2 on agricultural residues. Molasses substantially enhanced (63%) protease production (652.32 U/mL) than control (398.64 U/mL). Soybean meal supported maximum protease production (797.28 U/mL), followed by malt extract (770.1 U/mL), cotton cake (761.04 U/mL), gel
Braz. arch. biol. technol.. Publicado em: 2014-10
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9. Resistance training improves body composition and increases matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles of diet-induced obese rats
OBJECTIVE: We investigated the influence of resistance training on body composition and matrix metalloproteinase 2 activity in skeletal muscles of rats fed a high-fat diet. METHODS: Thirty-two Wistar rats were divided into four experimental groups (n = 8/each) according to diet and exercise status: Control (standard diet), Obese Control (high-fat diet)
Clinics. Publicado em: 2014-04
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10. Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae)
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractio
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 04/02/2014
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11. Expression of MMP-2 and MMP-9 in the rat trigeminal ganglion during the development of temporomandibular joint inflammation
Orofacial pain is a prevalent symptom in modern society. Some musculoskeletal orofacial pain is caused by temporomandibular disorders (TMDs). This condition has a multi-factorial etiology, including emotional factors and alteration of the masticator muscle and temporomandibular joints (TMJs). TMJ inflammation is considered to be a cause of pain in patients w
Braz J Med Biol Res. Publicado em: 18/11/2013
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12. Zimography is an effective method for detection of matrix metalloproteinase 2 (MMP-2) activity in cultured human fibroblasts
PURPOSE: To describe a method to characterize the gelatinase activity of cultured human periodontal fibroblasts stimulated with Pam3Cys and E. coli LPS, ligands of TLR2 and TLR4 respectively, and by centrifugation of the cultures, simulating an orthodontic force. METHODS: To study MMP-2 activity, primary cultures of human periodontal fibroblasts were stimula
Acta Cir. Bras.. Publicado em: 2013-03