Zymograms
Mostrando 1-12 de 49 artigos, teses e dissertações.
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1. Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganes
Braz. J. Microbiol.. Publicado em: 2016-06
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2. Increased expression of keratinase and other peptidases by Candida parapsilosis mutants
Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other
Braz J Med Biol Res. Publicado em: 2011-03
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3. Purification and biochemica characterisation of endoplasmic reticulum α 1,2-mannosidase from Sporothrix schenckiil
Alpha 1,2-mannosidases from glycosyl hydrolase family 47 participate in N-glycan biosynthesis. In filamentous fungi and mammalian cells, α1,2-mannosidases are present in the endoplasmic reticulum (ER) and Golgi complex and are required to generate complex N-glycans. However, lower eukaryotes such Saccharomyces cerevisiae contain only one α1,2-mannosidase i
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-02
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4. Enzimas de organismos aquÃticos e suas aplicaÃÃes fisiolÃgicas
In the last years, there has been a general increase in human demand for fishery products, followed by a significant increase in the world production of aquatic organisms. The growth of fishery industry generates, as consequence, large amounts of wastes and by-products rich in biomolecules, which are discharged into envioronment. Fishery wastes can be used i
Publicado em: 2009
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5. Efeito do hidrolisado protéico de camarão sobre as enzimas digestivas da tilápia do nilo (Oreochromis niloticus, L.)
Foi avaliada a influência de diferentes concentrações de hidrolisado protéico de camarão nas atividades enzimáticas dos intestinos de juvenis de tilápia do Nilo, e correlacionadas com parâmetros de crescimento (peso final, ganho de peso, ganho de peso diário, fator de crescimento específico, fator de conversão alimentar, fator de eficiência prot�
Publicado em: 2008
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6. Proteases digestivas do hepatopÃncreas dos camarÃes marinhos Farfantepenaeus subtilis e Farfantepenaeus paulensis
Farfantepenaeus subtilis and Farfantepenaeus paulensis are native shrimp species and appear as an alternative to Litopenaeus vannamei cultivation, which represents more than 90% of the farmed shrimp in the Northeast of Brazil. Proteases from hepatopancreas of F. subtilis juveniles and adults and F. paulensis juveniles were studied according to the following
Publicado em: 2008
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7. Caracterização da atividade tríptica do copépodo harpacticoida Tisbe biminiensis.
Tisbe biminiensis is a potential live prey for many species of aquatic animals since its nutritional value is better than those found in other feed organisms commonly used in crustacean and fish larvivultures. Live food seems to be a source of exogenous enzymes which eases food digestion of fish and crustacean early life stages. The aim of this work was to s
Publicado em: 2007
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8. Avaliação da ação bioinseticida de SBTI e vicilina de Erythrina velutina em enzimas digestivas e membrana peritrófica de larvas de Plodia interpunctella (Lepidoptera: Pyralidae)
Plodia interpunctella (Indian meal moth) is a cosmopolitan pest that attacks not only a wide range of stored grain as well other food products. Due to its economic importance several researches have focused in a method with ability to control this pest with few or no damage to the environment. The study of digestive enzymes inhibitors, lectins and chitin-bin
Publicado em: 2007
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9. Dehydrogenase Patterns in the Study of Bacteroidaceae1
Enzyme patterns were obtained by starch-gel electrophoresis of cell-free extracts from organisms in the family Bacteroidaceae. Esterases, phosphatases, and lactic and succinic dehydrogenases were detected but offered no possibility for classification purposes. Alpha-glycerophosphate dehydrogenase and 6-phosphogluconate dehydrogenase were not detected. Zymogr
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10. Methods for Visualization of Enzymes in Polyacrylamide Gels
White bands resulting from precipitation of dodecan-1-ol liberated by hydrolysis of sodium dodecyl sulfate and decan-5-ol released by hydrolysis of decan-5-yl sulfate produced zymograms of the primary and secondary alkylsulfatases from Pseudomonas C12B. Gas-liquid chromatographic analyses of ether extracts of the precipitate-containing segments of the zymogr
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11. Characterization of fibrinolytic activities of Treponema denticola.
Several fibrinolytic activities of Treponema denticola, an oral spirochete associated with gingivitis and periodontal disease, were identified and characterized following phase partitioning with the nonionic detergent Triton X-114. The apparent molecular masses of the proteases ranged from 91 to 228 kDa when analyzed in sodium dodecyl sulfate-polyacrylamide
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12. Simple, Sensitive Zymogram Technique for Detection of Xylanase Activity in Polyacrylamide Gels
A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against