Zygotes
Mostrando 1-12 de 192 artigos, teses e dissertações.
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1. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro
R. Bras. Zootec.. Publicado em: 30/09/2019
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2. Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
RESUMO O objetivo desse estudo foi avaliar a suplementação de meio de cultura de embriões com antioxidante obtido do extrato oleoso da Lippia origanoides no desenvolvimento e na qualidade de blastocistos produzidos in vitro. Oócitos coletados de ovários de matadouros foram maturados e fertilizados in vitro segundo procedimento laboratorial padrão. Zigo
Arq. Bras. Med. Vet. Zootec.. Publicado em: 14/06/2019
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3. Avaliação da Proporção Sexual de Embriões Desenvolvidos In Vitro e de Progênie a Campo de Touros Jovens. / Evaluation of the Sex Ratio of Embryos Developed In Vitro and in Field Progeny of Young Sires.
Some of the main problems that affect the in vitro production of embryos is the variation among bulls in relation to fertility and the greater birth of male embryos. Many factors can change the ratio 1:1 between the genders, both in the in vitro production of embryos and in the method of artificial insemination. To try to change this sex ratio in vitro, we s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/08/2011
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4. Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro / Different protocols to bovine donor preparation for in vitro embryo production
Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective of this study was to evaluate the effect of different synchronization of follicular wave protocols in the production of oocytes and embryos in vitro, of Bos taurus taurus and Bos
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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5. Different protocols to bovine donor preparation for in vitro embryo production / Protocolos hormonais de preparação de doadoras bovinas para produção de embriões in vitro
Efficiency of in vitro bovine embryo production has been affected by low quantity and quality of recovered oocytes from ultrasound-guided follicular aspiration (OPU). The objective of this study was to evaluate the effect of different synchronization of follicular wave protocols in the production of oocytes and embryos in vitro, of Bos taurus taurus and Bos
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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6. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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7. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offsp
Biology of Reproduction. Publicado em: 2011
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8. Influência da depleção e suplementação mitocondrial no processo de apoptose embrionária / Influence of mitochondrial depletion and suplementation in embryonic apoptosis
Studies developed with bovine zygotes submitted to a decrease of about 50% of the mitochondrial content did not present a deleterious effect on in vitro embryonic development. This model was developed by the centrifugation of zygotes after in vitro fertilization (IVF), removal of the mitochondria enriched cytoplast fraction (MECF) and posterior introduction
Publicado em: 2010
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9. Morphological differences in human zygotes and embryos cultured in different media / Efeito de quatro diferentes meios de cultura na qualidade morfologica de zigotos e embriões
Objective: compare the effects of four different culture media on the quality of zygotes and embryos. Methods: This retrospective study, performed at the Center for Human Reproduction of Campinas-Brazil analyzed 2289 embryos were assessed from September 2006 to September 2008. Long protocol was used for ovarian stimulation in all cases. The oocytes of each p
Publicado em: 2009
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10. Passagem celular, sexo e transcrição X-específica interferem no desenvolvimento embrionário e fetal de bovinos produzidos por transferência nuclear / Celular passage, sex and X-specific transcription interfere in bovine nuclear transfer embryo and fetal development
Cells cultured for long-term periods accumulate genetic and epigenetic modifications that result in improper nuclear reprogramming of somatic cell nuclear transfer (SCNT) embryos. Furthermore, the sex may be a limiting factor in blastocysts production and in post-implantation developmental competence. Therefore, the objective of this study was to determine t
Publicado em: 2007
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11. Avaliação da sensibilidade de zigotos murinos à Brucella abortus para o estabelecimento de um modelo experimental em estudos de interações embriões-patógenos / Sensibility evaluation of mouse zygotes to Brucella abortus for establishment of an experimental model to pathogen-embryo interaction studies
Os objetivos desse trabalho foram avaliar in vitro a sensibilidade de zigotos murinos à Brucella abortus, assim como a eficácia das lavagens seqüenciais e do tratamento com tripsina, padronizados pela International Embryo Transfer Society, na sua remoção e/ou inativação, a fim de estabelecer um modelo acessível para o estudo de interações embriões
Publicado em: 2005
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12. Transferência gênica mediada por espermatozóides para geração de jundiás (Rhamdia quelen) transgênicos / Sperm Mediated Gene Transfer for generation of transgenic jundiás (Rhamdia quelen)
Various methodologies to insert exogenous DNA into eukaryotic cells have been studied in the last two decades. In fish, the technique most studied to the moment is microinjection, which promotes the insertion of fragments of interest genes into zygotes. However, this methodology has some negative aspects: it is time and work consuming, requires qualified lab
Publicado em: 2004