Zfn
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. An update on the tools for creating transgenic animal models of human diseases – focus on atherosclerosis
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing technique
Braz J Med Biol Res. Publicado em: 25/04/2019
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2. From Gene Targeting to Genome Editing: Transgenic animals applications and beyond
RESUMOTecnologias de modificação do genoma são ferramentas poderosas para a biologia molecular e áreas afins. Avanços na transgênese animal e em tecnologias de edição do genoma durante as últimas três décadas permitiram interrogar de modo sistemático a função de genes, o que pode ajudar a compreender como o genoma influencia a fisiologia celula
An. Acad. Bras. Ciênc.. Publicado em: 2015-08
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3. Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases
Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this
Genet. Mol. Biol.. Publicado em: 2014
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4. Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
Targeted mutagenesis is an essential tool of reverse genetics that could be used experimentally to investigate basic plant biology or modify crop plants for improvement of important agricultural traits. Although targeted mutagenesis is routine in several model organisms including yeast and mouse, efficient and widely usable methods to generate targeted modif
National Academy of Sciences.