Whole Cell Patchclamp
Mostrando 1-12 de 520 artigos, teses e dissertações.
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1. O ß-hidroxibutirato modifica os processos de ativação e inativação dos canais para sódio e potássio em corpos celulares de neurônios dos gânglios das raizes dorsais de ratos.
Under physiological conditions, the ketone bodies -hydroxybutyrate, acetoacetate and acetone are products of -oxidation of fatty acids that occurs in hepatocytes in response to hypoglycemia. This process occurs during fasting, high fat diets (such as the ketogenic diet, used in cases of drug-resistant epilepsy) and during diabetes mellitus in order to meet t
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/12/2011
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2. Modulação da atividade dos canais de sódio dependentes de voltagem e de canais TRPV1 por (-)-Carvona
Among the natural products with experimentally proven analgesic properties, the monoterpernes are of great importance, recognized as the main chemical constituents of essential oils from aromatic plants. ()-Carvone is an example of monoterpene with antinociceptive properties, founded as the main active constituent of oils from some species of the genus Menth
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/12/2011
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3. Ação antiespasmódica do trans-cariofileno e o bloqueio de canais para Ca2+ em músculo liso traqueal de rato. / Antispasmodic action of the trans-caryophyllene and blocking Ca2 + channels in rat tracheal smooth muscle.
Trans-cariofileno é um constituinte importante em várias espécies de óleos essenciais de plantas medicinais usadas na medicina popular do Brasil, pertence à classe química dos sesquiterpenos e tem sido submetido a vários estudos. Aqui, o efeito deste constituinte foi avaliado no músculo liso da via aérea. As atividades biológicas do trans-cariofile
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/03/2010
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4. Investigação do efeito vasorelaxante e caracterização eletrofisiológica dos alcalóides curina e reticulina
It was been demonstrated that curine and reticuline, induced a vasodilator effect in the rat small mesenteric arteries through inhibition of voltage-gated Ca2+ channels (VGCC). These compounds, curine and reticuline were isolated from the root barks of Chondrondendron platyphyllum and Ocotea duckei Vattimo, respectively, therefore the aim of this work was to
Publicado em: 2009
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5. Purification, Biochemical and Electrophysiological Characterization of the Toxin Mic6c7NTX from the Micrurus ibiboboca (Merrem, 1820) / Purificação, caracterização bioquímica e eletrofisiológica da toxina Mic6c7NTX da Peçonha da Serpente Micrurus ibiboboca (Merrem, 1820)
Snake venoms contain a complex arsenal of protein bio-active components, many of these being neurotoxins (NTXs). These snakes have high neurotoxic activity venom, corresponding to the Elapidae family, which includes coral snakes (Micrurus) whose venom contains circa 90-95% of low molecular mass protein components. Among these, several are postsynaptic neurot
Publicado em: 2008
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6. Modulation of intercellular communication by differential regulation and heteromeric mixing of co-expressed connexins
Intercellular communication may be regulated by the differential expression of subunit gap junction proteins (connexins) which form channels with differing gating and permeability properties. Endothelial cells express three different connexins (connexin37, connexin40, and connexin43) in vivo. To study the differential regulation of expression and synthesis o
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-04
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7. Potassium currents of isolated Necturus enterocytes: a whole-cell patch-clamp study.
1. The whole-cell recording mode of the patch-clamp technique was used to investigate the ionic currents of enterocytes isolated from the small intestine epithelium of Necturus maculosus. 2. When enterocytes were bathed in a physiological Ringer solution and dialysed with a K(+)-rich intracellular solution containing 1.5 x 10(-7) M intracellular Ca2+, strong
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8. Neuronal modulation of calcium channel activity in cultured rat astrocytes.
The patch-clamp technique was used to study whether cocultivation of neurons and astrocytes modulates the expression of calcium channel activity in astrocytes. Whole-cell patch-clamp recordings from rat brain astrocytes cocultured with rat embryonic neurons revealed two types of voltage-dependent inward currents carried by Ca2+ and blocked by either Cd2+ or
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9. A patch-clamp study of mammalian platelets and their voltage-gated potassium current.
1. Mammalian platelets were freshly isolated from human, rabbit, or rat blood. The whole-cell and cell-attached voltage-clamp variations of the patch-clamp technique were employed to study the passive electrical properties and ion channels of unstimulated platelets. 2. The input capacitance of a platelet measured by the phase-sensitive detection method was a
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10. Patch-clamp recording of amino acid-activated responses in "organotypic" slice cultures.
Patch-clamp recording techniques were used to study the properties of amino acid-activated channels in cultured "organotypic" slices from rat cerebellum and hippocampus. Hippocampal pyramidal cells responded to the three main glutamatergic agonists, N-methyl-D-aspartate (N-Me-D-Asp), quisqualate, and kainate, whereas Purkinje cells responded only to quisqual
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11. Mechanoelectrical transducer has discrete conductances in the chick vestibular hair cell.
Properties of mechanoelectrical transduction were studied at the single-cell level by applying a whole-cell recording variation of the patch-clamp technique to dissociated vestibular hair cells of chicks. The hair bundle was directly stimulated by a glass rod, and transduction currents were recorded from the cell body. After a triangular movement of the stim
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12. Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits
Physiological properties of central nervous system neurons infected with a pseudorabies virus were examined in vitro by using whole-cell patch-clamp techniques. A strain of pseudorabies virus (PRV 152) isogenic with the Bartha strain of PRV was constructed to express an enhanced green fluorescent protein (EGFP) from the human cytomegalovirus immediate early
The National Academy of Sciences.