Vmax
Mostrando 25-36 de 1570 artigos, teses e dissertações.
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25. Chemical modification of Aspergillus nigerβ-glucosidase and its catalytic properties
Aspergillus niger β-glucosidase was modified by covalent coupling to periodate activated polysaccharides (glycosylation). The conjugated enzyme to activated starch showed the highest specific activity (128.5 U/mg protein). Compared to the native enzyme, the conjugated form exhibited: a higher optimal reaction temperature, a lower Ea (activation energy), a h
Braz. J. Microbiol.. Publicado em: 2015-03
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26. Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate
Braz. arch. biol. technol.. Publicado em: 13/01/2015
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27. Cinética de absorção de nitrogênio e acúmulo de frações solúveis nitrogenadas e açúcares em girassol
O girassol (Helianthus annuus L.) tem se destacado como cultura promissora, dentre as oleaginosas com importância agroenergética. Considerando-se os nutrientes exigidos pela cultura do girassol, o nitrogênio é o que mais limita a produção, além de afetar seu rendimento de óleo. Este trabalho objetivou avaliar aspectos do metabolismo e a cinética de
Pesqui. Agropecu. Trop.. Publicado em: 2014-12
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28. Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii
A lipase from the endophytic fungus Cercospora kikuchii was purified, biochemically characterized and the effects of spray drying on stabilization of the purified enzyme were studied. The lipase was purified 9.31-fold with recovery of 26.6% and specific activity of 223.6 U/mg. The optimum pH and temperature were 4.6 and 35 ºC, respectively, while the Vmax w
Braz. J. Chem. Eng.. Publicado em: 2014-12
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29. Estratégias de seleção assistida por marcadores para desenvolvimento de plantas de soja resistentes ao nematoide de cisto
A seleção assistida por marcadores (SAM) permite identificarlinhagens resistentes com base em alelos de marcadores genéticos ligados aocaráter, o que reduz o número de linhagens avaliados fenotipicamente, uma daslimitações ao desenvolvimento de cultivares resistentes ao nematoide de cistoda soja (NCS). Neste trabalho objetivou-se avaliar a eficiência
Crop Breed. Appl. Biotechnol.. Publicado em: 2014-10
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30. Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35
Braz. arch. biol. technol.. Publicado em: 29/08/2014
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31. The comparison of urodynamic findings ?n women with various types of urinary ?ncontinence
PurposeWe aimed to determine the differences of the urodynamic findings of mix urinary incontinence (MUI), urge urinary incontinence (UUI), and stress urinary incontinence (SUI), and to evaluate the urodynamic findings in different groups by using bladder sensitivity index (BSI).Materials and MethodsThe data of 99 patients who underwent urodynamic testing re
Int. braz j urol.. Publicado em: 2014-04
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32. Megazol and its bioisostere 4H-1,2,4-triazole: comparing the trypanocidal, cytotoxic and genotoxic activities and their in vitro and in silico interactions with the Trypanosoma brucei nitroreductase enzyme
Megazol (7) is a 5-nitroimidazole that is highly active against Trypanosoma cruzi and Trypanosoma brucei, as well as drug-resistant forms of trypanosomiasis. Compound 7 is not used clinically due to its mutagenic and genotoxic properties, but has been largely used as a lead compound. Here, we compared the activity of 7 with its 4H-1,2,4-triazole bioisostere
Mem. Inst. Oswaldo Cruz. Publicado em: 18/03/2014
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33. Desempenho da cultura da soja influenciada pela época de dessecação de Urochloa ruziziensis e pressões de pastejo
O sistema integração lavoura-pecuária tem demonstrado várias vantagens econômicas e ambientais. No entanto, há carência de informações sobre os efeitos da pressão de pastejo de Urochloa ruziziensis e de épocas de dessecação sobre o desempenho da cultura da soja em sucessão. O objetivo desta pesquisa foi avaliar o desempenho de três cultivares
Rev. Ciênc. Agron.. Publicado em: 2014
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34. Kinetic characterization of glucose aerodehydrogenase from Aspergillus niger EMS-150-F after optimizing the dose of mutagen for enhanced production of enzyme
In the present study enhanced production of glucose aerodehydrogenase from Aspergillus niger has been achieved after optimizing the dose of chemical mutagen ethyl methane sulfonate (EMS) that has not been reported earlier. Different doses of mutagen were applied and a strain was developed basing upon the best production. The selected strain Aspergillus niger
Braz. J. Microbiol.. Publicado em: 2013-12
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35. An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: downstream processing and biochemical characterization
An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum te
Braz. J. Microbiol.. Publicado em: 2013-12
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36. Detection of 1-aminocyclopropane-1-carboxylate oxidase activity in seeds of Stylosanthes humilis H.B.K
The activity of 1-aminocyclopropane-1-carboxylate oxidase (ACO) was characterized in seeds of the tropical legume Townsville stylo (Stylosanthes humilis) both in vitro (desalted extract of non-dormant seeds) and in vivo (entire dormant seeds). Optimum conditions for maximum in vitro ACO activity in a Trizma-HCl 100 mM buffered medium were: pH 7.0, temperatur
Theor. Exp. Plant Physiol.. Publicado em: 2013