Ureagenesis
Mostrando 1-10 de 10 artigos, teses e dissertações.
-
1. GLUCONEOGÉNESIS HEPÁTICA EN OVINOS (Ovis aries) ALIMENTADOS CON UNA DIETA ALTA EN NITRÓGENO NO PROTEICO
La adición en la dieta de nitrógeno no proteico (NNP) aumenta la ureagénesis afectando a la gluconeogénesis hepática por reducción en la conversión de propionato a glucosa in vitro (Overton et al 1999), sin embargo los experimentos in vivo son contradictorios (Fernández et al 1988, Choung y Chamberlain 1995, Mutsvangwa et al 1997, Fernández et al 20
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/05/2006
-
2. Glutamine and glutamate as vital metabolites
Glucose is widely accepted as the primary nutrient for the maintenance and promotion of cell function. This metabolite leads to production of ATP, NADPH and precursors for the synthesis of macromolecules such as nucleic acids and phospholipids. We propose that, in addition to glucose, the 5-carbon amino acids glutamine and glutamate should be considered to b
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-02
-
3. Differential expression of the two human arginase genes in hyperargininemia. Enzymatic, pathologic, and molecular analysis.
Previous studies in our laboratory and others have demonstrated in humans and other mammals two isozymes of arginase (AI and AII) that differ both electrophoretically and antigenically. AI, a cytosolic protein found predominantly in liver and red blood cells, is believed to be chiefly responsible for ureagenesis and is the one missing in hyperargininemic pat
-
4. Arginine, an indispensable amino acid for patients with inborn errors of urea synthesis.
The role of arginine as an essential amino was evaluated in four children with one of the deficiencies of carbamyl phosphate synthetase, ornithine transcarbamylase, argininosuccinate synthetase, and argininosuccinase. Within 15-68 h after arginine deprivation nitrogen accumulated as ammonium or glutamine or both, but glutamine was quantitatively the largest
-
5. Growth hormone regulates amino acid transport in human and rat liver.
Human growth hormone (GH) has been shown to improve nitrogen balance in surgical patients and to decrease urea production. This has been thought to be due primarily to an increase in protein synthesis in skeletal muscle. Little attention has focused on the liver as a possible site where GH may modulate amino acid uptake and thereby divert nitrogen away from
-
6. Glycine and urea kinetics in nonalcoholic steatohepatitis in human: effect of intralipid infusion
The rates of oxidation of glycine and ureagenesis were quantified in the basal state and in response to an intravenous infusion of intralipid with heparin (IL) in healthy subjects (n = 8) and in subjects with nonalcoholic steatohepatitis (NASH) (n = 6). During fasting, no significant difference in weight-specific rate of appearance (Ra) of glycine, glycine o
American Physiological Society.
-
7. An arginine to glutamine mutation in residue 109 of human ornithine transcarbamylase completely abolishes enzymatic activity in Cos1 cells.
Ornithine transcarbamylase (OTC) is an important enzyme in the detoxification of ammonia to urea, and its deficiency is the most common inborn error of ureagenesis in humans. Among 24 cases of OTC deficiency previously examined, three unrelated individuals all showed loss of a Taq I site in the OTC gene corresponding to codon 109, suggesting that this Taq I
-
8. In vivo nitrogen metabolism in ornithine transcarbamylase deficiency.
We developed a new technique that monitors metabolic competency in female heterozygotes for ornithine transcarbamylase deficiency (OTCD). The method uses mass spectrometry to measure conversion of (15)NH4Cl to [15N]urea and [5-(15)N]glutamine following an oral load of (15)NH4Cl. We found that heterozygotes converted significantly less NH3 nitrogen to urea, w
-
9. Failure of the Normal Ureagenic Response to Amino Acids in Organic Acid-loaded Rats: PROPOSED MECHANISM FOR THE HYPERAMMONEMIA OF PROPIONIC AND METHYLMALONIC ACIDEMIA
Propionic and methylmalonic acidemia are both known to be associated with hyperammonemia. Rats injected with 10 or 20 mmol/kg of propionate or 20 mmol/kg of methylmalonate, along with 1.5 g/kg of a mixture of amino acids, developed severe hyperammonemia, whereas rats administered the same dosages of acetate did not. In vitro, neither propionyl nor methylmalo
-
10. Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver.
OBJECTIVE: Metabolic activity of transformed human liver (Hep G2) cells and primary rat hepatocytes were compared during in vitro application of a gel entrapment bioartificial liver. BACKGROUND: Clinical trials of bioartificial liver devices containing either transformed liver cells or primary hepatocytes have been initiated. A study comparing transformed li