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Mostrando 1-12 de 37 artigos, teses e dissertações.
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1. Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
Abstract INTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated th
Rev. Soc. Bras. Med. Trop.. Publicado em: 05/09/2019
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2. Fracionamento de zinco em amostras de leite / Zinc fractionation in milk samples
Neste trabalho foram feitos estudos voltados ao preparo de amostras e à especiação química dos teores de zinco presentes em amostras de diferentes tipos de leite (bovinos, ovelha, cabra e origem vegetal (soja). A proposta visou estabelecer as diferentes proporções dos teores de zinco existentes em proteínas das amostras procurando-se observar a biodis
Publicado em: 2009
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3. UtilizaÃÃo de tÃcnicas de eletroforese em gel de poliacrilamida na identificaÃÃo da adiÃÃo de leite de vaca ao leite de cabra / Detection of cowâs milk and goatâs milk mixtures by polyacrylamide gel electrophoresis techniques
O presente trabalho foi conduzido com o intuito de avaliar mÃtodos eletroforÃticos para detecÃÃo de fraudes pela adiÃÃo de leite de vaca ao leite de cabra, incluindo sua aplicaÃÃo na rotina dos laboratÃrios de anÃlise de alimentos, definiÃÃo das condiÃÃes de amostragem e o preparo das amostras para anÃlise. Os mÃtodos avaliados foram tÃcnica
Publicado em: 2003
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4. Estudos cineticos, fisico-quimicos e conformacional da fosfatase acida de sementes de mamona (Ricinus communis)
This work contains results about kinetic, physico-chemical and conformationalstudies of castor bean seed acid phosphatase. About kinetic approaches we studied the effect of inhibitors and determined the amino acids present in the active site. Inorganic phosphate, molybidate and o vanadate were competitive inhibitors, with Ki values of 0,14 mM, 10 x 10-6 mM e
Publicado em: 2001
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5. Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor
Abnormal production of interferon alpha (IFN-a) has been found in certain autoimmune diseases and can be also observed after prolonged therapy with IFN-a. IFN-a can contribute to the pathogenesis of allograft rejection in bone marrow transplants. Therefore, the development of IFN-a inhibitors as a soluble receptor protein may be valuable for the therapeutic
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
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6. Ghost protein damage by peroxynitrite and its protection by melatonin
We have studied the effect of peroxynitrite (ONOO-) on the membrane cytoskeleton of red blood cells and its protection by melatonin. Analysis of the protein fraction of the preparation by SDS-PAGE revealed a dose-dependent (0-600 µM ONOO-) disappearance at pH 7.4 of the main proteins: spectrin, band 3, and actin, with the concomitant formation of high-molec
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-01
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7. THE ACTION OF CERTAIN DIURETICS ON THE FUNCTION OF THE KIDNEY AS MEASURED BY THE UREA CLEARANCE TEST
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8. Production of antibody to individual polypeptides derived from purified hepatitis B surface antigen.
Purified preparations of hepatitis B surface antigen (HBsAg) were solubilized with sodium dodecyl sulfate and urea under reducing conditions and subsequently fractionated by preparative sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis (PAGE). Pools of the individual fractions eluted from the preparative PAGE were concentrated and purified furth
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9. Electrophoretic Characterization of a Detergent-Treated Plasma Membrane Fraction from Corn Roots 1
Experiments were conducted to determine conditions essential for electrophoretic characterization of a detergent-extracted plasma membrane fraction from corn (Zea mays L.) roots. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) initially gave poor resolution of polypeptides in the plasma membrane fraction and, upon detergent treatment f
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10. Induction of Barley Leaf Urease 1
Foliar urea application on barley plants increased leaf urease activity for 5 hours with a peak of 20-fold at 2 hours. To discern the mode of urease induction, urea with or without inhibitors and [35S]methionine were incubated with leaf sections for different lengths of time. Urease was extracted, partially purified, electrophoresed, and then quantified by f
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11. Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica.
A toxin with dermonecrotic activity (DNT) was purified from sonic extracts of Bordetella bronchiseptica L3 of pig origin at phase I by chromatographic and electrophoretic methods. The purification procedure was one developed for obtaining the Pasteurella multocida DNT from sonic extracts with some modifications. Dermonecrotizing activity of B. bronchiseptica
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12. Attempts to restore scrapie prion infectivity after exposure to protein denaturants.
A wealth of experimental evidence argues that infectious prions are composed largely, if not entirely, of the scrapie isoform of the prion protein. We attempted to restore scrapie infectivity after exposure to protein denaturants including urea, chaotropic salts, and SDS. None of the procedures restored infectivity. Dialysis to remove slowly chaotropic ions