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Mostrando 25-36 de 848 artigos, teses e dissertações.
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25. Double mutation of Saccharomyces cerevisiae for enhanced β-D-fructofuranosidase fructohydrolase productivity and application of growth kinetics for parametric significance analysis
Abstract The kinetics of an extracellular β-D-fructofuranosidase fructohydrolase production by Saccharomyces cerevisiae in a chemically defined medium, i.e., sucrose peptone agar yeast extract at pH 6, was investigated. The wild-type was treated with a chemical mutagen, methyl methane sulfonate. Among the six mutants isolated, methyl methane sulfonate-V was
Braz. J. Microbiol.. Publicado em: 2016-03
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26. Value addition of corn husks through enzymatic production of xylooligosaccharides
ABSTRACT Corn husks are the major wastes of corn industries with meagre economic significance. The present study was planned for value addition of corn husk through extraction of xylan, followed by its enzymatic hydrolysis into xylooligosaccharides, a pentose based prebiotic. Compositional analysis of corn husks revealed neutral detergent fibre 68.87%, acid
Braz. arch. biol. technol.. Publicado em: 2016
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27. The genotypic diversity and lipase production of some thermophilic bacilli from different genera
Abstract Thermophilic 32 isolates and 20 reference bacilli were subjected to Rep-PCR and ITS-PCR fingerprinting for determination of their genotypic diversity, before screening lipase activities. By these methods, all the isolates and references could easily be differentiated up to subspecies level from each other. In screening assay, 11 isolates and 7 refer
Braz. J. Microbiol.. Publicado em: 2015-12
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28. Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil
Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the c
Braz. J. Microbiol.. Publicado em: 27/10/2015
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29. Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions
A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke collected from Shihezi (Xinjiang, China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an
Aspergillus niger strain by analysis of 16S rRNA. To improve inulinase production, this fungus was subjected to mutagenesiBraz. J. Microbiol.. Publicado em: 2015-09
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30. Characterization of pectinase activity for enology from yeasts occurring in Argentine Bonarda grape
Pectinolytic enzymes are greatly important in winemaking due to their ability to degrade pectic polymers from grape, contributing to enhance process efficiency and wine quality. This study aimed to analyze the occurrence of pectinolytic yeasts during spontaneous fermentation of Argentine Bonarda grape, to select yeasts that produce extracellular pectinase
Braz. J. Microbiol.. Publicado em: 2015-09
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31. Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36
The aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon
Braz. arch. biol. technol.. Publicado em: 2015-08
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32. STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION
Abstract Penicillium echinulatum S1M29 is a mutant with cellulase and xylanase production comparable to the most studied microorganisms in the literature. However, its potential to produce these enzymes has not been fully investigated. This study aimed at optimizing salt and urea concentrations in the mineral solution, employing the response surface methodol
Braz. J. Chem. Eng.. Publicado em: 2015-03
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33. RECOVERY OF CYCLODEXTRIN GLUCANOTRANSFERASE (CGTase) USING IMMOBILIZED METAL CHELATING AFFINITY CHROMATOGRAPHY
Abstract Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activit
Braz. J. Chem. Eng.. Publicado em: 2015-03
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34. Copper induction and differential expression of laccase in Aspergillus flavus
Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chro
Braz. J. Microbiol.. Publicado em: 2015-03
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35. Characterization of xylanase from Streptomyces sp. FA1 and its application for bamboo hydrolysis
Native cellulose can be disintegrated into substructures with nano-size dimension through electronspinning. Typically, the most common source material available for cellulose nano-fiber production is wood. More attempts are employed to find new suitable resources to produce nano-cellulose at present. Bamboo boasts the abundant resource in China and possesses
Matéria (Rio J.). Publicado em: 2014-12
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36. Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum
Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL afte
Braz. arch. biol. technol.. Publicado em: 2014-12