Two Dimensional Differential Gel Electrophoresis
Mostrando 1-12 de 68 artigos, teses e dissertações.
-
1. Proteomic analysis of halotolerant proteins under high and low salt stress in Dunaliella salina using two-dimensional differential in-gel electrophoresis
Abstract Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins u
Genet. Mol. Biol.. Publicado em: 13/05/2016
-
2. Differencial proteome of clear-cell renal cell carcinoma (ccRCC) tissues
PurposeWe attempted to detect, for the first time in a Brazilian cohort, differences in protein expression between clear-cell renal cell carcinoma (ccRCC) and their normal adjacent tissues, aiming to identify biomarkers and/or therapeutic target candidates for this disease.Material and MethodsTwenty-four ccRCC and adjacent normal tissues were collected after
Int. braz j urol.. Publicado em: 2013-01
-
3. Busca de biomarcadores para esquizofrenia em plaquetas utilizando eletroforese diferencial em gel bidimensional (2D-DIGE) e espectrometria de massas / Search for schizophrenia biomarkers in platelets using two dimensional differential gel electrophoresis and mass spectrometry analysis
A esquizofrenia é uma doença crônica, grave e incapacitante que afeta cerca 24 milhões de pessoas em âmbito mundial. É caracterizada por uma desorganização no pensamento que prejudica a funcionalidade do indivíduo. Existem intervenções que são efetivas e contribuem para a diminuição da prevalência do transtorno, pois ajudam o portador a levar
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/08/2011
-
4. ProspecÃÃo de proteÃnas envolvidas no amadurecimento pÃs-colheita da graviola (Annona muricata L.) / Prospecting for proteins involved in post-harvest ripening of soursop (Annona muricata L.)
The soursop (Annona muricata L.) fruit is widely used in food, because it provides vitamins, carbohydrates, proteins, lipids and other molecules for human nutrition. In recent years, soursop (exotic tropical fruit) has had a prominent position in the Brazilian economy, especially for northeastern states such as, Ceara, Alagoas, Bahia and Pernambuco. The high
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/08/2011
-
5. Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds
The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccat
Brazilian Archives of Biology and Technology. Publicado em: 2011-06
-
6. Análise proteômica da embriogênese somática e da aquisição de competência embriogência de Ocotea catharinensis Mez (Lauraceae)
Ocotea catharinensis is a forest tree species from Mata Atlântica that has economic importance due to its wood and essential oils. O. catharinensis became an endangered species mostly because of its intense exploitation and its low seed production and viability. The objective of this work was to study O. catharinensis somatic embryogenesis through the compa
Publicado em: 2006
-
7. Expressão diferencial de Xanthomonas axonopodis pv. citri na interação com Citrus sinensis utilizando eletroforese bidimensional de proteinas e cDNA RDA ("Representational Difference Analysis")
The present study reports the differential expression of X. axonopodis pv. citri in response to different growth conditions by two-dimensional gel electrophoresis (2-DE) of proteins and cDNA RDA (Representational Difference Analysis). For the protein analysis, the bacterium was cultured in the basal medium MM1 and in the presence of leaf extracts from a susc
Publicado em: 2002
-
8. Proteomics Analysis by Two-Dimensional Differential Gel Electrophoresis Reveals the Lack of a Broad Response of Neisseria meningitidis to In Vitro-Produced AI-2
To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling
American Society for Microbiology.
-
9. Enhanced resolution of DNA restriction fragments: a procedure by two-dimensional electrophoresis and double-labeling.
A probe-free method was developed to detect DNA rearrangement in bacteria based on the electrophoretic separation of twice-digested restriction fragments of genomic DNA into a two-dimensional (2-D) pattern. The first restriction enzyme digestion was done in solution, followed by electrophoresis of the restriction fragments in one dimension. A second restrict
-
10. Differential synthesis of polypeptides during morphogenesis of Mucor.
The extent of differential gene expression during morphogenesis of Mucor racemosus was investigated by two-dimensional polyacrylamide gel electrophoresis of neutral and acidic polypeptides. Cellular proteins were labeled with [35S]methionine in cells growing in either the yeast or hyphal form, or in yeast cells undergoing the transition of hyphae. The result
-
11. Plasma proteome of severe acute respiratory syndrome analyzed by two-dimensional gel electrophoresis and mass spectrometry
We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four patients with SARS in different time courses, and a total of 38 differential spots were selected for protein identification. Most of the proteins identified are acute
National Academy of Sciences.
-
12. Isolation of Mycoplasma pulmonis membranes and identification of surface antigens.
Labeling of Mycoplasma pulmonis cells by iodination with the Bolton-Hunter reagent was shown to efficiently label membrane-associated proteins without significant loss of viability. Labeled proteins proven to be surface exposed by differential proteolytic digestion were analyzed by autoradiography of two-dimensional polyacrylamide gel electrophoresis (PAGE),