Tswv
Mostrando 13-24 de 27 artigos, teses e dissertações.
-
13. Métodos de aplicação e dosagem de thiamethoxam no controle de tripes em tomate
O tripes Frankliniella schultzei Trybom (Thysanoptera: Thripidae) é um dos mais importantes vetores de tomato spotted wilt tospovirus (TSWV) na cultura do tomate no Brasil. Dois métodos de aplicação do inseticida thiamethoxam foram comparados no controle de tripes em tomate. O experimento foi conduzido em delineamento de blocos ao acaso com sete tratamen
Horticultura Brasileira. Publicado em: 2003-09
-
14. Erigeron bonariensis: hospedeira alternativa do Lettuce mosaic virus no Brasil
O gênero Erigeron (Asteraceae), de plantas da vegetação espontânea, encontra-se amplamente disseminado nas regiões Sul e Sudeste do Brasil, sendo freqüentemente encontrado em lavouras perenes e anuais. Plantas de E. bonariensis com sintoma de mosaico, típico do induzido por vírus, foram coletadas no município de São Paulo e submetidas a análises a
Fitopatologia Brasileira. Publicado em: 2003-06
-
15. Caracterização do Tomato chlorotic spot virus isolado de jiló no Vale do Paraíba, Estado de São Paulo
Os tospovírus são responsáveis por perdas significativas em diversas culturas, principalmente solanáceas. No município de São José dos Campos (SP), plantas de jiló (Solanum gilo) apresentando sintomas de mosaico, bolhosidades, nanismo e queda acentuada da produção foram coletadas para análise. Visando a caracterização do agente causador dos sint
Fitopatologia Brasileira. Publicado em: 2002-06
-
16. Expression and Characterization of a Soluble Form of Tomato Spotted Wilt Virus Glycoprotein GN
Tomato spotted wilt virus (TSWV), a member of the Tospovirus genus within the Bunyaviridae, is an economically important plant pathogen with a worldwide distribution. TSWV is transmitted to plants via thrips (Thysanoptera: Thripidae), which transmit the virus in a persistent propagative manner. The envelope glycoproteins, GN and GC, are critical for the infe
American Society for Microbiology.
-
17. Expression of a viral polymerase-bound host factor turns human cell lines permissive to a plant- and insect-infecting virus
Tospoviruses are the only plant-infecting members of the Bunyaviridae family of ambisense ssRNA viruses. Tomato spotted wilt tospovirus (TSWV), the type-member, also causes mild infection on its main insect vector, Frankliniella occidentalis. Herein, we identified an F. occidentalis putative transcription factor (FoTF) that binds to the TSWV RNA-dependent RN
National Academy of Sciences.
-
18. The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins
The nonstructural NSm protein of tomato spotted wilt tospovirus (TSWV) represents a putative viral movement protein involved in cell-to-cell movement of nonenveloped ribonucleocapsid structures. To study the molecular basis of NSm function, we expressed the protein in Escherichia coli and investigated protein–protein and protein–RNA interactions of NSm p
The National Academy of Sciences.
-
19. Alfalfa Mosaic Virus RNAs Serve as Cap Donors for Tomato Spotted Wilt Virus Transcription during Coinfection of Nicotiana benthamiana
Tomato spotted wilt virus (TSWV) was shown to use alfalfa mosaic virus (AMV) RNAs as cap donors in vivo during a mixed infection in Nicotiana benthamiana. By use of nested reverse transcription-PCR, TSWV N and NSs mRNAs provided with capped leader sequences derived from all four AMV RNAs could be cloned and sequenced. The sequence specificity of the putative
American Society for Microbiology.
-
20. In vivo analysis of the TSWV cap-snatching mechanism: single base complementarity and primer length requirements
Requirements for capped leader sequences for use during transcription initiation by tomato spotted wilt virus (TSWV) were tested using mutant alfalfa mosaic virus (AMV) RNAs as specific cap donors in transgenic Nicotiana tabacum plants expressing the AMV replicase proteins. Using a series of AMV RNA3 mutants modified in either the 5′-non-translated region
Oxford University Press.
-
21. The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis
Tospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT
American Society for Microbiology.
-
22. Viral genetic determinants for thrips transmission of Tomato spotted wilt virus
Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently trans
National Academy of Sciences.
-
23. Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions
Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these plants were infected with Tomato spotted wilt virus (TSWV), a plant-infecting member of the Bunyaviridae. Infection with TSWV resulted in complete reactivation of GFP expression, similar to the case for Potato virus Y, but di
American Society for Microbiology.
-
24. Tomato Spotted Wilt Virus Glycoproteins Exhibit Trafficking and Localization Signals That Are Functional in Mammalian Cells
The glycoprotein precursor (G1/G2) gene of tomato spotted wilt virus (TSWV) was expressed in BHK cells using the Semliki Forest virus expression system. The results reveal that in this cell system, the precursor is efficiently cleaved and the resulting G1 and G2 glycoproteins are transported from the endoplasmic reticulum (ER) to the Golgi complex, where the
American Society for Microbiology.