Trypanosome Cruzi
Mostrando 25-36 de 38 artigos, teses e dissertações.
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25. The Anti-Influenza Virus Drug Rimantadine Has Trypanocidal Activity
We report here that bloodstream forms of the African trypanosome, Trypanosoma brucei, are sensitive to the anti-influenza virus drug rimantadine (50% inhibitory concentration of 1.26 μg ml−1 at pH 7.4). The activity is pH dependent and is consistent with a mechanism involving inhibition of the ability to regulate internal pH. Rimantadine is also toxic to
American Society for Microbiology.
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26. Specific inhibition of Trypanosoma cruzi neuraminidase by the human plasma glycoprotein "cruzin".
Plasma of normal human individuals was shown to contain an inhibitor of Trypanosoma cruzi neuraminidase (NAase; acylneuraminyl hydrolase, sialidase, EC 3.2.1.18). The inhibitor has been purified to homogeneity by PEG precipitation, CM Affi-Gel Blue Sepharose chromatography, and gel filtration. The purified preparation inhibits T. cruzi NAase at a concentrati
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27. Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum
In most aerobic organisms hemoperoxidases play a major role in H2O2-detoxification, but trypanosomatids have been reported to lack this activity. Here we describe the properties of an ascorbate-dependent hemoperoxidase (TcAPX) from the American trypanosome Trypanosoma cruzi. The activity of this plant-like enzyme can be linked to the reduction of the parasit
The National Academy of Sciences.
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28. In vitro 3' end processing and poly(A) tailing of RNA in Trypanosoma cruzi.
Pre-mRNA in kinetoplastids is processed to maturity following unique pathways requiring a transplicing event that links a common 39 nucleotide leader to the 5' termini of the mature mRNAs. The mechanisms of this reaction and other steps of mRNA processing; i.e., 5' capping and 3' cleavage and polyadenylation, have not been resolved. Herein, we describe a 3'
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29. Antibody Maturation in Trypanosoma cruzi-Infected Rats
The study of antibody avidity changes during infection has improved the understanding of the pathologic processes involved in several infectious diseases. In some infections, like toxoplasmosis, this information is being used for diagnostic purposes. Results of the evolution of antibody avidity for different specific antigens in Trypanosome cruzi-infected ra
American Society for Microbiology.
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30. Transforming Growth Factor α Binds to Trypanosoma cruzi Amastigotes To Induce Signaling and Cellular Proliferation
Macrophages secrete transforming growth factor α (TGF-α) to trigger proliferation of cancer cells. Here, we report a new role for TGF-α in modulating the direct cellular proliferation of a parasitic protozoan, Trypanosoma cruzi. Amastigotes present two classes of receptors for TGF-α with different binding affinities. 125I-TGF-α binding was competed by a
American Society for Microbiology.
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31. In vitro trypanocidal activities of new S-adenosylmethionine decarboxylase inhibitors.
A series of novel aromatic derivatives based on the structure of methylglyoxal bis(guanylhydrazone) (MGBG) was examined for in vitro antitrypanosomal activities and cytotoxicities for human cells. One-third of the compounds tested showed trypanocidal activity at concentrations below 0.5 microM after an incubation period of 72 h. Structure-activity analysis r
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32. Molecular cloning of mtp70, a mitochondrial member of the hsp70 family.
We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes a previously unidentified member of the 70-kilodalton heat shock protein (hsp70) family. Among all the eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure to the hsp70 of Escherichia coli, DnaK, which func
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33. Characterization of a cDNA encoding a cysteine-rich cell surface protein located in the flagellar pocket of the protozoan Trypanosoma brucei.
We have characterized a cDNA encoding a cysteine-rich, acidic integral membrane protein (CRAM) of the parasitic protozoa Trypanosoma brucei and Trypanosoma equiperdum. Unlike other membrane proteins of T. brucei, which are distributed throughout the cell surface, CRAM is concentrated in the flagellar pocket, an invagination of the cell surface of the trypano
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34. Comparison of the genes coding for the common 5' terminal sequence of messenger RNAs in three trypanosome species.
Messenger RNAs of Trypanosoma brucei share a common 5' terminal sequence of 35 nucleotides, encoded by a mini-exon located in 1.35-kb tandemly linked repeats. We show here that sequences, almost identical to the mini-exon of T. brucei, are present in mRNAs from members of two other kinetoplastid subgenera: Trypanosoma vivax and Trypanosoma cruzi. As in T. br
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35. Vitamin C biosynthesis in trypanosomes: A role for the glycosome
The capacity to synthesize vitamin C (ascorbate) is widespread in eukaryotes but is absent from humans. The last step in the biosynthetic pathway involves the conversion of an aldonolactone substrate to ascorbate, a reaction catalyzed by members of an FAD-dependent family of oxidoreductases. Here we demonstrate that both the African trypanosome, Trypanosoma
National Academy of Sciences.
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36. Macrophage activation by cord factor (trehalose 6,6'-dimycolate): enhanced association with and intracellular killing of Trypanosoma cruzi.
Cord factor (trehalose 6,6'-dimycolate[TDM] ), a mixture of 6,6'-diesters of alpha, alpha-D-trehalose with natural mycolic acids, has been described as having immunoregulatory and antitumor activities in vivo, although the relevant mechanisms of action remain unelucidated. In this work, we measured the effects of TDM on both mouse macrophage association with