Transduced Keratinocytes
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Desenvolvimento de modelos animais de terapia gênica para o hormônio de crescimento utilizando queratinócitos transduzidos e injeção direta de DNA plasmidial / Development of animal models of growth hormone gene therapy using transduced keratinocytes and direct injection of naked DNA
Queratinócitos são células bastante atrativas para a transferência gênica ex vivo e liberação sistêmica, uma vez que as proteínas secretadas por estas células podem atingir a circulação via um mecanismo similar ao processo natural. No presente trabalho, queratinócitos transduzidos mediante um vetor retroviral com o gene do hormônio de crescimen
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/12/2010
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2. Secreção de hormônio de crescimento de camundongo por queratinícitos humanos primários: perspectivas para um modelo animal de terapia gênica cutânea / SECRETION OF MOUSE GROWTH HORMONE BY TRANSDUCED PRIMARY HUMAN KERATINOCYTES: PROSPECTS FOR AN ANIMAL MODEL OF CUTANEOUS GENE THERAPY
Keratinocytes are a very attractive vehicle for ex vivo gene transfer and systemic delivery, since proteins secreted by these cells may reach the circulation via a mechanism which mimics the natural process. An efficient retroviral vector (LXSN) encoding the mouse growth hormone gene (mGH) was used to transduce primary human keratinocytes. Organotypic raft c
Publicado em: 2008
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3. β1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms
In keratinocytes, the β1 integrins mediate adhesion to the extracellular matrix and also regulate the initiation of terminal differentiation. To explore the relationship between these functions, we stably infected primary human epidermal keratinocytes and an undifferentiated squamous cell carcinoma line, SCC4, with retroviruses encoding wild-type and mutant
The American Society for Cell Biology.
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4. Alternative Fates of Keratinocytes Transduced by Human Papillomavirus Type 18 E7 during Squamous Differentiation
The human papillomavirus type 18 (HPV-18) E7 protein promotes S-phase reentry in postmitotic, differentiated keratinocytes in squamous epithelium to facilitate vegetative viral DNA amplification. To examine the nature and fate of the differentiated cells that reenter S phase, organotypic cultures of primary human keratinocytes transduced with HPV-18 E7 were
American Society for Microbiology.
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5. In Vivo Assessment of Gene Delivery to Keratinocytes by Lentiviral Vectors
For skin gene therapy, introduction of a desired gene into keratinocyte progenitor or stem cells could overcome the problem of achieving persistent gene expression in a significant percentage of keratinocytes. Although keratinocyte stem cells have not yet been completely characterized and purified for gene targeting purposes, lentiviral vectors may be superi
American Society for Microbiology.
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6. Keratinocyte gene therapy for systemic diseases. Circulating interleukin 10 released from gene-transferred keratinocytes inhibits contact hypersensitivity at distant areas of the skin.
This study has examined the systemic effects of a circulating gene product, human interleukin 10 (IL-10), released from transduced keratinocytes. IL-10 is an anti-inflammatory cytokine which has an inhibitory effect on contact hypersensitivity (CHS). An expression vector (phIL-10) was constructed for human IL-10 and was injected into the dorsal skin of hairl
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7. Topical colchicine selection of keratinocytes transduced with the multidrug resistance gene (MDR1) can sustain and enhance transgene expression in vivo
For skin gene therapy, achieving prolonged high-level gene expression in a significant percentage of keratinocytes (KC) is difficult because we cannot selectively target KC stem cells. We now demonstrate that topical colchicine treatment can be used to select, in vivo, KC progenitor cells transduced with the multidrug resistance gene (MDR1). When human skin
The National Academy of Sciences.
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8. Clonal analysis of stably transduced human epidermal stem cells in culture.
We have transduced normal human keratinocytes with retroviral constructs expressing a bacterial beta-galactosidase (beta-gal) gene or a human interleukin-6 (hIL-6) cDNA under control of a long terminal repeat. Efficiency of gene transfer averaged approximately 50% and 95% of clonogenic keratinocytes for beta-gal and hIL-6, respectively. Both genes were stabl
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9. In vivo restoration of laminin 5 β3 expression and function in junctional epidermolysis bullosa
The blistering disorder, lethal junctional epidermolysis bullosa (JEB), can result from mutations in the LAMB3 gene, which encodes laminin 5 β3 (β3). Appropriate expression of LAMβ3 in JEB skin tissue could potentially ameliorate the symptoms of the underlying disease. To explore the utility of this therapeutic approach, primary keratinocytes from si
The National Academy of Sciences.
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10. A Two-Stage, p16INK4A- and p53-Dependent Keratinocyte Senescence Mechanism That Limits Replicative Potential Independent of Telomere Status†
With increasing frequency during serial passage in culture, primary human keratinocytes express p16INK4A (p16) and undergo senescence arrest. Keratinocytes engineered to express hTERT maintain long telomeres but typically are not immortalized unless, by mutation or other heritable event, they avoid or greatly reduce p16 expression. We have confirmed that ker
American Society for Microbiology.
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11. Defects in transforming growth factor-β signaling cooperate with a Ras oncogene to cause rapid aneuploidy and malignant transformation of mouse keratinocytes
Genetic inactivation of the transforming growth factor-β (TGF-β) signaling pathway can accelerate tumor progression in the mouse epidermal model of multistage carcinogenesis. By using an in vitro model of keratinocyte transformation that parallels in vivo malignant conversion to squamous cell carcinoma, we show that v-rasHa transduced primary TGF-β1−/�
The National Academy of Sciences.
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12. Physiological and retinoid-induced proliferations of epidermis basal keratinocytes are differently controlled
To investigate the roles of retinoic acid (RA) receptors (RARs) in the physiology of epidermis that does not express RARβ, conditional spatio-temporally controlled somatic mutagenesis was used to selectively ablate RARα in keratinocytes of RARγ-null mice. Keratinocyte proliferation was maintained in adult mouse epidermis lacking both RARα and RARγ, as w
Oxford University Press.