Trace Alignment
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
ABSTRACT Newcastle disease (ND) is a major infectious disease of the poultry caused by a virulent strain of Avian Paramyxovirus - 1, that is a single strand non-segmented negative sense RNA virus. ND virus is major threat to the poultry industry in many countries of the world. The study was aimed to isolate and identify Newcastle disease virus (NDV) by using
Braz. arch. biol. technol.. Publicado em: 27/06/2016
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2. Técnicas para compreensão de rastros de execução de programas orientados a objetos
Várias abordagens para facilitar a compreensão do comportamento de sistemas de software têm sido propostas. Mudanças perfectivas em sistemas de software bem estabelecidos são mais fáceis de executar quando a equipe de desenvolvimento tem um entendimento sólido do código fonte. Mas é razoável assumir que o uso de um sistema de código aberto para in
Publicado em: 2011
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3. Basecalling with LifeTrace
A pivotal step in electrophoresis sequencing is the conversion of the raw, continuous chromatogram data into the actual sequence of discrete nucleotides, a process referred to as basecalling. We describe a novel algorithm for basecalling implemented in the program LifeTrace. Like Phred, currently the most widely used basecalling software program, LifeTrace t
Cold Spring Harbor Laboratory Press.
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4. Automated detection of point mutations using fluorescent sequence trace subtraction.
The final step in the detection of mutations is to determine the sequence of the suspected mutant and to compare it with that of the wild-type, and for this fluorescence-based sequencing instruments are widely used. We describe some simple algorithms forcomparing sequence traces which, as part of our sequence assembly and analysis package, are proving useful
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5. ESPript/ENDscript: extracting and rendering sequence and 3D information from atomic structures of proteins
The fortran program ESPript was created in 1993, to display on a PostScript figure multiple sequence alignments adorned with secondary structure elements. A web server was made available in 1999 and ESPript has been linked to three major web tools: ProDom which identifies protein domains, PredictProtein which predicts secondary structure elements and NPS@ wh
Oxford University Press.
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6. Behavior of DNA fibers stretched by precise meniscus motion control
A modified DNA combing method, which can precisely locate straightened DNA fibers on a substrate, has been developed. Precise motion control of a DNA solution droplet on hydrophobic surfaces has allowed detailed analyses of DNA straightening behavior. Our method provides a technique for consistently straightening λ phage DNA on a trace of droplet motion, th
Oxford University Press.
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7. Elasmobranchs express separate cholecystokinin and gastrin genes
The peptide hormone gastrin was long believed to be specific for higher vertebrates, whereas its homologue, cholecystokinin (CCK), has been assumed to represent the original ancestor of the CCK/gastrin family. To trace the divergence of the CCK/gastrin family beyond birds, reptiles, and amphibians we have now examined sharks. Distinct CCK and gastrin peptide
The National Academy of Sciences of the USA.
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8. A Strategy to Retrieve the Whole Set of Protein Modules in Microbial Proteomes
Protein homology is often limited to long structural segments that we have previously called modules. We describe here a suite of programs used to catalog the whole set of modules present in microbial proteomes. First, the Darwin AllAll program detects homologous segments using thresholds for evolutionary distance and alignment length, and another program cl
Cold Spring Harbor Laboratory Press.
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9. Isomaltulose Synthase from Klebsiella sp. Strain LX3: Gene Cloning and Characterization and Engineering of Thermostability
The gene (palI) encoding isomaltulose synthase (PalI) from a soil bacterial isolate, Klebsiella sp. strain LX3, was cloned and characterized. PalI converts sucrose into isomaltulose, trehalulose, and trace amounts of glucose and fructose. Sequence domain analysis showed that PalI contains an α-amylase domain and (β/α)8-barrel structures, suggesting that i
American Society for Microbiology.
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10. DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca.
Two genes encoding cellulases E1 and E4 from Thermomonospora fusca have been cloned in Escherichia coli, and their DNA sequences have been determined. Both genes were introduced into Streptomyces lividans, and the enzymes were purified from the culture supernatants of transformants. E1 and E4 were expressed 18- and 4-fold higher, respectively, in S. lividans