Toxoids
Mostrando 1-12 de 67 artigos, teses e dissertações.
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1. Production and characterization of recombinant chimeras C and D of Clostridium botulinum / Produção e caracterização de quimeras recombinantes C e D de Clostridium botulinum
Bovine Botulism is a lethal intoxication caused by the ingestion of the neurotoxins produced by Clostridium botulinum types C and D that inhibit the release of acetylcholine at the neuromuscular junction leading to death by flaccid paralysis. It produces important economic losses, being a major cause of casualties in cattle in several regions of Brazil. The
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/08/2012
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2. Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA
Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostrid
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2010
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3. Study of the potential of adjuvants toxoids Stx1 and Stx2 of Escherichia coli on native outer membrane vesicle preparations of Neisseria meningitidis B in BALB/c mice / Estudo do potencial adjuvante dos toxóides Stx1 e Stx2 de Escherichia coli em preparações com antígenos de vesículas de membrana externa de Neisseria meningitidis B em camundongos BALB/c
As vacinas antimeningocócicas têm se demonstrado efetivas contra os sorogrupos A e C, no entanto ainda não existe vacina contra o sorogrupo B devido à similaridade entre a estrutura capsular do polissacáride B e o ácido polisiálico que faz parte do tecido cerebral humano, podendo levar à autoimunidade. O objetivo deste estudo foi investigar as propri
Publicado em: 2009
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4. Genotyping of Clostridium perfringens associated with sudden death in cattle
Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small in
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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5. Evaluation of adjuvants in new triple bacterial vaccine formulation. / Avaliação de adjuvantes em novas formulações de vacina tríplice bacteriana.
The whole cell pertussis vaccines present some reactogenicity and the acellular, less reactogenic, have prohibitive use due to its high cost. Instituto Butantan developed a less reactogenic whole cell pertussis vaccine (Plow), with low lipopolysaccharide content and an acellular vaccine (Pa), by simple and economic methodology. These preparations, combined t
Publicado em: 2009
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6. Study of irradiated bothropstoxin-1 with60Co gamma rays: immune system behavior
Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved im
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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7. Avaliação da eficiência de vacinas contra Clostridium septicum
Twelve commercial vaccines against clostridiosis were evaluated having in their composition Clostridium septicum toxoids and/or bacterial cells, have been evaluated for potency by mice serum neutralization tests using rabbit or bovine sera and challenge test in guinea-pig. The vaccines, coded as T1, T10 and T11, elicited alpha antitoxin titers in rabbits whi
Publicado em: 2003
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8. Development of a purified cholera toxoid. III. Refinements in purification of toxin and methods for the determination of residual somatic antigen.
The addition of an ultrafiltration step to the purification procedures previously described for cholera toxin (Rappaport et al., (1974) permitted the preparation of highly purified antigenic toxoids essentially free of somatic antigen(s). The purity of such toxoids is established: (i) by the absence of more than about one part limulus amebocyte lysate (LAL)-
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9. Development of a Purified Cholera Toxoid II. Preparation of a Stable, Antigenic Toxoid by Reaction of Purified Toxin with Glutaraldehyde
Evidence is presented which confirms that cholera toxoids obtained by reaction of purified toxin with Formalin possess the ability to partially reactivate both in vivo and in vitro. At the same time, conditions are presented for the preparation of stable, antigenic cholera toxoids by reaction of purified toxin with glutaraldehyde. Treatment of purified chole
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10. Toxoid of Clostridium botulinum type F: purification and immunogenicity studies.
Toxin from Clostridium botulinum type F was recovered from dialysis cultures and partially purifed by: (i) ammonium sulfate and ethanol precipitation; (ii) O-(diethylaminoethyl)-cellulose chromatography; or (iii) diethylaminoethyl-cellulose chromatography followed by O-(carboxymethyl)-cellulose chromatography. Toxin purities as reflected by specific activity
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11. Toxoids of Pseudomonas aeruginosa exotoxin-A: photoaffinity inactivation of purified toxin and purified toxin derivatives.
For the preparation of greatly detoxified but highly immunogenic toxoids, two enzymatically active, low-toxicity derivatives of Pseudomonas aeruginosa exotoxin-A were further inactivated by photoaffinity labeling. These derivatives were formed during toxin purification, when a relatively crude toxin preparation was concentrated by ammonium sulfate precipitat
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12. Pertussis toxin-induced alterations of murine hepatic drug metabolism following administration of diphtheria and tetanus toxoids and pertussis vaccine adsorbed.
Administration of pertussis toxin (PT) in combination with diphtheria and tetanus toxoids adsorbed (DT vaccine) or with acellular pertussis vaccine adsorbed and diphtheria and tetanus toxoids (APDT) elicits dose- and time-dependent alterations in hepatic drug metabolism in mice. Cytochrome P-450 (P-450) levels were inhibited more than 50% at 7 days following