Tissue Culture
Mostrando 37-48 de 3946 artigos, teses e dissertações.
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37. DNA damage and primordial follicle activation after in vitro culture of sheep ovarian cortex in Morus nigra leaf extract
RESUMO: Este estudo avaliou o efeito do extrato das folhas de Morus nigra, com ou sem suplementos, sobre a morfologia, a ativação e o dano ao DNA de folículos pré-antrais cultivados inclusos em tecido ovariano. Os ovários foram coletados e divididos em fragmentos, sendo um fixado para análise histológica e ensaio de marcação de terminações dUTP me
Pesq. Vet. Bras.. Publicado em: 2019-01
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38. Propagación del banano: técnicas tradicionales, nuevas tecnologías e innovaciones
Abstract The different propagation systems for the banana: conventional or by tissue culture are reviewed. Conventional propagation, mainly using suckers, whole rhizomes or bits are still in use for familiar plantings or in farms for local markets in many tropical countries, while vitroplants are the rule in modern commercial banana cultivation. After exami
Rev. Bras. Frutic.. Publicado em: 17/09/2018
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39. A broad-range PCR technique for the diagnosis of infective endocarditis
Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A
Braz. J. Microbiol.. Publicado em: 2018-09
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40. Bioprospecting and selection of growth-promoting bacteria for Cymbidium sp. orchids
ABSTRACT: Inoculants containing bacteria which promote growth in plants can increase productivity and both the economic and the environmental cost in plant crop systems. Similarly, in the flower and ornamental plant sector, the use of diazotrophic bacteria is a promising approach for improving orchid propagation from tissue culture to the ex vitro environmen
Sci. agric. (Piracicaba, Braz.). Publicado em: 2018-09
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41. The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi
Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficu
Braz. J. Microbiol.. Publicado em: 2018-06
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42. Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concent
Braz J Med Biol Res. Publicado em: 28/05/2018
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43. Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides
Abstract Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/
Acta Cir. Bras.. Publicado em: 2018-05
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44. Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion
Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on h
Braz J Med Biol Res. Publicado em: 26/03/2018
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45. Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for
Braz. J. Microbiol.. Publicado em: 2018-03
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46. Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm
Abstract Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental c
J. Appl. Oral Sci.. Publicado em: 22/02/2018
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47. Complement system contributes to modulate the infectivity of susceptible TcI strains of Trypanosoma cruzi
BACKGROUND Trypanosoma cruzi is a protozoan parasite and an etiological agent of Chagas disease. There is a wide variability in the clinical outcome of its infection, ranging from asymptomatic individuals to those with chronic fatal mega syndromes. Both parasite and host factors, as well as their interplay, are thought to be involved in the process. OBJECT
Mem. Inst. Oswaldo Cruz. Publicado em: 19/02/2018
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48. Culture of human dental pulp cells at variable times post-tooth extraction
Abstract The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was t
Braz. oral res.. Publicado em: 01/02/2018