Thymidine Kinase
Mostrando 1-12 de 1252 artigos, teses e dissertações.
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1. Vias de sinalização intracelular envolvidas no efeito proliferativo de relaxina em células de sertoli / Intracelular signaling pathways involved in the proliferative effect of relaxin in rat Sertoli cells
Relaxin is an insulin-related peptide that activates the G-protein coupled receptor RXFP1. Although relaxin plays an important role in female reproduction its physiological role in the male reproductive system is still unclear. We have previously demonstrated that relaxin and RXFP1 are expressed in testis (FILONZI et al., 2007; CARDOSO et al., 2010). Both re
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2011
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2. Selection and characterization of brivudin resistant bovine herpesvirus type 5
Bovine herpesvirus type 5 (BoHV-5) is the agent of meningoencephalitis, an important disease of cattle in South America. The neuropathogenesis of BoHV-5 infection is poorly understood and most previous research focused on the role of envelope glicoproteins in neurovirulence. Thymidine kinase (TK) is a viral enzyme necessary for virus replication in neurons a
Brazilian Journal of Microbiology. Publicado em: 2010-03
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3. Construction and growth properties of bovine herpesvirus type 5 recombinants defective in the glycoprotein E or thymidine kinase gene or both
Bovine herpesvirus type 5 (BoHV-5) is an important pathogen of cattle in South America. We describe here the construction and characterization of deletion mutants defective in the glycoprotein E (gE) or thymidine kinase (TK) gene or both (gE/TK) from a highly neurovirulent and well-characterized Brazilian BoHV-5 strain (SV507/99). A gE-deleted recombinant vi
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-02
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4. A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 s
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-02
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5. ATTENUATION AND IMMUNOGENICITY OF A RECOMBINANT BOVINE HERPESVIRUS 5 DEFECTIVE IN GLYCOPROTEIN E AND THYMIDINE KINASE / ATENUAÇÃO E IMUNOGENICIDADE DE UMA CEPA RECOMBINANTE DO HERPESVÍRUS BOVINO TIPO 5 DEFECTIVA NA GLICOPROTEÍNA E E ENZIMA TIMIDINA QUINASE
O presente trabalho descreve a atenuação/virulência e imunogenicidade de uma cepa recombinante do herpesvírus bovino tipo 5 (BoHV-5) candidata a cepa vacinal. O recombinante BoHV-5gE/TKΔ foi construído a partir da cepa brasileira SV507/99 e contém deleções nos genes da glicoproteína E (gE) como marcador antigênico - e da enzima timidina quinas
Publicado em: 2010
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6. Epidemiologia molecular do vírus da laringotraqueíte infecciosa isolados de surtos em poedeiras comerciais no Estado de São Paulo / Molecular epidemiology of Infectious laryngotracheitis vírus isolated from an outbreak in commercial layer flocks in São Paulo State
The objective of this study was the molecular detection and characterization of field isolates of infectious laryngotracheitis virus (ILTV) detected from commercial chickens with and without clinical signs of the disease from regions of the São Paulo state. The study included samples collected during and after of the first epidemic infectious laryngotrachei
Publicado em: 2009
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7. INFECÇÃO EXPERIMENTAL DE COELHOS COM RECOMBINANTES DO HERPESVÍRUS BOVINO TIPO 5 DEFECTIVOS NOS GENES DA TIMIDINA QUINASE E DA GLICOPROTEÍNA E / EXPERIMENTAL INFECTION OF RABBITS WITH BOVINE HERPESVIRUS 5 RECOMBINANTS DEFECTIVE IN THYMIDINE KINASE AND GLYCOPROTEIN E GENES
O herpesvírus bovino tipo 5 (BoHV-5), agente da meningoencefalite herpética bovina, possui grande importância na América do Sul e tem motivado pesquisas para o desenvolvimento de vacinas eficazes e seguras. Essa dissertação relata a investigação da virulência em coelhos de três recombinantes do BoHV-5, candidatos vacinais, contendo deleções nos g
Publicado em: 2009
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8. Construction and evaluation of plasmid expressing thymidine kinase suicide gene and immunomodulatory evolved interleukin-12 gene for medullary thyroid carcinoma gene therapy / Construção e avaliação da ação de plasmídio contendo gene suicida timidina quinase e gene imunomodulador da interleucina 12 otimizada, visando terapia gênica para carcinoma medular de tireóide
Os tratamentos convencionais para carcinoma medular de tireóide (CMT) metastático são insatisfatórios. Tanto a quimioterapia quanto a radioterapia são pouco eficazes para a doença avançada. Portanto, a terapia gênica é uma promissora opção. Trabalhos de construção de vetores plasmidiais ou adenovirais específicos para cultura de células de car
Publicado em: 2007
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9. Characterization of Brazilian isolates of equine herpesvirus type A / Caracterização genomica de isolados brasileiros do herpesvirus equino do tipo 1
Equine Herpesvirus Type 1 (EHV-1) is a member of Alphaherpesvirinae subfamily implicated with abortions, respiratory and neurological disturbs in horses. The principal mode of viral transmission is through close contact virus-containing secretions of infected horses. Systemic pathogenesis in which this virus is implicated combines primary respiratory infecti
Publicado em: 2005
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10. An Adenovirus Vector Containing the Suicide Gene Thymidine Kinase for a Broad Application in Cancer Gene Therapy
Treatment of cancer using gene therapy is based on adding a property to the cell leading to its elimination. One possibility is the use of suicide genes that code for enzymes that transform a pro-drug into a cytotoxic product. The most extensively used is the herpes simplex virus thymidine kinase (TK) gene, followed by administration of the antiviral drug ga
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-06
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11. Effects of lipopolysaccharide on low- and high-density cultured rabbit vascular smooth muscle cells: differential modulation of nitric oxide release, ERK1/ERK2 MAP kinase activity, protein tyrosine phosphatase activity, and DNA synthesis
Previous studies have shown that exogenously generated nitric oxide (NO) inhibits smooth muscle cell proliferation. In the present study, we stimulated rabbit vascular smooth muscle cells (RVSMC) with E. coli lipopolysaccharide (LPS), a known inducer of NO synthase transcription, and established a connection between endogenous NO, phosphorylation/dephosphory
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-02
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12. Thymidine uptake, thymidine incorporation, and thymidine kinase activity in marine bacterium isolates.
One assumption made in bacterial production estimates from [3H]thymidine incorporation is that all heterotrophic bacteria can incorporate exogenous thymidine into DNA. Heterotrophic marine bacterium isolates from Tampa Bay, Fla., Chesapeake Bay, Md., and a coral surface microlayer were examined for thymidine uptake (transport), thymidine incorporation, the p