Thermophilic Behavior
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Thermogenesis and post-prandial termophly in Bothrops moojeni (Serpentes: Viperidae) in captivity / Termofilia e termogênese pós-prandiais em Bothrops moojeni (Serpentes:Viperidae) em cativeiro
The study of the preferred temperatures and thermophilic behavior is essential to understand the snakes thermal biology. Although some studies have been reported, most of them are focused in temperate and diurnal species. Thus, little is known about the tropical and sub-tropical nocturnal species. In the present study , a video-monitored arena with a thermal
Publicado em: 2009
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2. Psychrophilic, mesophilic, and thermophilic triosephosphate isomerases from three clostridial species.
Triosephosphate isomerase was purified to homogeneity as judged by analytical gel electrophoresis from clostridium sp. strain 69, clostridium pasteurianum, and C. thermosaccharolyticum, which grow optimally at 18, 37, and 55 C, respectively. Comparative studies on these purified proteins showed that they had the same molecular weight (53,000) and subunit mol
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3. Characterization and Purification of an Aldose Reductase from the Acidophilic and Thermophilic Red Alga Galdieria sulphuraria.
The acidophilic and thermophilic red alga Galdieria sulphuraria is able to grow heterotrophically on at least six different pentoses. These pentoses are reduced in the cell to pentiols by an NADP-dependent aldose reductase. The pentiols are then introduced into the oxidative pentose phosphate pathway via NAD-dependent polyol dehydrogenases and pentulokinases
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4. Light-induced motility of thermophilic Synechococcus isolates from Octopus Spring, Yellowstone National Park.
This study demonstrates light-induced motility of two thermophilic Synechococcus isolates that are morphologically similar but that belong to different cyanobacterial lineages. Both isolates migrated away from densely inoculated streaks to form fingerlike projections extending toward or away from the light source, depending on the light intensity. However, t
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5. Thermodynamics of the binding of Thermus aquaticus DNA polymerase to primed-template DNA
DNA binding of the Type 1 DNA polymerase from Thermus aquaticus (Taq polymerase) and its Klentaq large fragment domain have been studied as a function of temperature. Equilibrium binding assays were performed from 5 to 70°C using a fluorescence anisotropy assay and from 10 to 60°C using isothermal titration calorimetry. In contrast to the usual behavior of
Oxford University Press.
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6. A Mechanism for Precision-Sensing via a Gradient-Sensing Pathway: A Model of Escherichia coli Thermotaxis
Thermotaxis is the phenomenon where an organism directs its movement toward its preferred temperature. So far, the molecular origin for this precision-sensing behavior remains a puzzle. We propose a model of Escherichia coli thermotaxis and show that the precision-sensing behavior in E. coli thermotaxis can be carried out by the gradient-sensing chemotaxis p
The Biophysical Society.
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7. Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.
The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The gene was transcribed, presumably from its o
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8. Control of oligomeric enzyme thermostability by protein engineering.
The ability to control the resistance of an enzyme to inactivation due to exposure to elevated temperatures is essential for the understanding of thermophilic behavior and for developing rational approaches to enzyme stabilization. By means of site-directed mutagenesis, point mutations have been engineered in the dimeric enzyme yeast triosephosphate isomeras
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9. A model for the cooperative free energy transduction and kinetics of ATP hydrolysis by F1-ATPase
Although the binding change mechanism of rotary catalysis by which F1-ATPase hydrolyzes ATP has been supported by equilibrium, kinetic, and structural observations, many questions concerning the function remain unanswered. Because of the importance of this enzyme, the search for a full understanding of its mechanism is a key problem in structural biology. Ma
National Academy of Sciences.