The Chitin Binding Proteins
Mostrando 1-12 de 41 artigos, teses e dissertações.
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1. Alternative splicing originates different domain structure organization of Lutzomyia longipalpis chitinases
BACKGROUND The insect chitinase gene family is composed by more than 10 paralogs, which can codify proteins with different domain structures. In Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil, a chitinase cDNA from adult female insects was previously characterized. The predicted protein contains one catalytic domain and one chiti
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-02
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2. Propriedades BioquÃmicas e Funcionais de uma ProteÃna Ligante à Quitina Purificada de Sementes de Moringa oleifera Lamarck / Biochemical and Functional Properties of Chitin-Binding Protein Purified from seeds of Moringa oleifera Lamarck
Moringa oleifera Lam. à uma planta originÃria do Noroeste da Ãndia, bem adaptada Ãs regiÃes tropicais. De suas sementes foi isolada uma nova proteÃna ligante à quitina, a Mo-CBP3, com propriedades coagulantes e atividade antifÃngica contra o fitopatÃgeno Fusarium solani. As proteÃnas foram extraÃdas da farinha delipidada de sementes com o tampÃo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/12/2009
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3. Determinação de motivos de ligação à quitina em vicilinas de Canavalia ensiformis e Vigna unguiculata através de métodos in silico e relação com suas toxicidades para o bruquídeo Callosobruchus maculatus (Coleoptera:Bruchidae)
Chitin is an important structural component of the cellular wall of fungi and exoskeleton of many invertebrate plagues, such as insects and nematodes. In digestory systems of insects it forms a named matrix of peritrophic membrane. One of the most studied interaction models protein-carbohydrate is the model that involves chitin-binding proteins. Among the in
Publicado em: 2009
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4. Characterization of chitinase gene from lutzomyia longipalpis:alternative splicing description and search for promoter sequence / Caracterização de gene de quitinase de Lutzomyia longipalpis: descrição de processamento alternativo e busca por seqüência promotora
Leishmaniasis is a disease caused by Leishmania protozoa transmitted by the bite of infected sand flies. Current methods used to combat this illness have been shown to be inefficient, and better knowledge of aspects related to Leishmania-sand fly interaction are necessary for the development of new controlling methods. A cDNA codifying for a midgutspecific c
Publicado em: 2009
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5. PurificaÃÃo, caracterizaÃÃo e avaliaÃÃo da atividade fÃngica da Lectina de CladÃnias de Opuntia fÃcus indica
Lectins are hemagglutinating proteins with carbohydrate binding sites; these proteins have biological properties including antimicrobial activity. The aims of this work were the isolation of Opuntia ficus indica lectin (OfÃL) and evaluation of its antifungal activity. Hemagglutinating activity (HA) of cladode crude extract (CE20%) was evaluated using rabbit
Publicado em: 2008
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6. Avaliação da ação bioinseticida de SBTI e vicilina de Erythrina velutina em enzimas digestivas e membrana peritrófica de larvas de Plodia interpunctella (Lepidoptera: Pyralidae)
Plodia interpunctella (Indian meal moth) is a cosmopolitan pest that attacks not only a wide range of stored grain as well other food products. Due to its economic importance several researches have focused in a method with ability to control this pest with few or no damage to the environment. The study of digestive enzymes inhibitors, lectins and chitin-bin
Publicado em: 2007
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7. Study of the insecticide and pro-inflammatory activity of lectin isolated from annona coriacea mart seeds / Estudo da atividade inseticida e pro-inflamatoria da lectina isolada de sementes de Annona coriacea Mart
Lectins are a group of proteins and/or glycoproteins, which exhibit specific and reversible carbohydrate-binding activities. Studies have demonstrated that such proteins possess important biological activities including insecticide activity as well as immunological and physiological responses in animals. In this investigation, our aim was to compare the effe
Publicado em: 2006
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8. Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein
Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of an open reading frame (ORF) of 2067 nucleotides with codes f
Genetics and Molecular Biology. Publicado em: 2005-12
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9. Induction of Chitin-Binding Proteins during the Specific Attachment of the Marine Bacterium Vibrio harveyi to Chitin
Previous work has shown that attachment of Vibrio harveyi to chitin is specific and involves at least two chitin-binding peptides. However, the roles and regulation of these chitin-binding peptides in attachment are still unclear. Here we show that preincubation with the oligomeric sugars composing chitin stimulated chitinase activity, cellular attachment to
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10. Role of Chitin-Binding Proteins in the Specific Attachment of the Marine Bacterium Vibrio harveyi to Chitin
We examined the mechanism of attachment of the marine bacterium Vibrio harveyi to chitin. Wheat germ agglutinin and chitinase bind to chitin and competitively inhibited the attachment of V. harveyi to chitin, but not to cellulose. Bovine serum albumin and cellulase do not bind to chitin and had no effect on bacterial attachment to chitin. These data suggest
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11. Identification of a Chitin-Binding Protein Secreted by Pseudomonas aeruginosa
One of the major proteins secreted by Pseudomonas aeruginosa is a 43-kDa protein, which is cleaved by elastase into smaller fragments, including a 30-kDa and a 23-kDa fragment. The N-terminal 23-kDa fragment was previously suggested as corresponding to a staphylolytic protease and was designated LasD (S. Park and D. R. Galloway, Mol. Microbiol. 16:263–270,
American Society for Microbiology.
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12. Molecular Analysis of the Gene Encoding a Novel Chitin-Binding Protease from Alteromonas sp. Strain O-7 and Its Role in the Chitinolytic System
Alteromonas sp. strain O-7 secretes several proteins in response to chitin induction. We have found that one of these proteins, designated AprIV, is a novel chitin-binding protease involved in chitinolytic activity. The gene encoding AprIV (aprIV) was cloned in Escherichia coli. DNA sequencing analysis revealed that the open reading frame of aprIV encoded a
American Society for Microbiology.