Thapsigargin
Mostrando 1-12 de 221 artigos, teses e dissertações.
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1. Pharmacological characterization of the calcium influx pathways involved in nitric oxide production by endothelial cells
RESUMO Objetivo: Caracterizar as vias do influxo de cálcio envolvidas no aumento sustentado da concentração intracelular de cálcio na célula endotelial, essencial para a síntese e a liberação de fatores relaxantes. Métodos: Analisamos o efeito de derivados pirazólicos sintetizados recentemente, descritos como inibidores seletivos para canais ORAI
Einstein (São Paulo). Publicado em: 03/06/2019
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2. Successful application of virtual screening and molecular dynamics simulations against antimalarial molecular targets
The main challenge in the control of malaria has been the emergence of drug-resistant parasites. The presence of drug-resistant Plasmodium sp. has raised the need for new antimalarial drugs. Molecular modelling techniques have been used as tools to develop new drugs. In this study, we employed virtual screening of a pyrazol derivative (Tx001) against four ma
Mem. Inst. Oswaldo Cruz. Publicado em: 10/11/2016
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3. β-Citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea
β-Citronellol is an alcoholic monoterpene found in essential oils such Cymbopogon citratus (a plant with antihypertensive properties). β-Citronellol can act against pathogenic microorganisms that affect airways and, in virtue of the popular use of β-citronellol-enriched essential oils in aromatherapy, we assessed its pharmacologic effects on the contracti
Braz J Med Biol Res. Publicado em: 04/12/2015
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4. Structure-based drug design studies of the interactions ofent-kaurane diterpenes derived from Wedelia paludosa with the Plasmodium falciparumsarco/endoplasmic reticulum Ca2+-ATPase PfATP6
Malaria is responsible for more deaths around the world than any other parasitic disease. Due to the emergence of strains that are resistant to the current chemotherapeutic antimalarial arsenal, the search for new antimalarial drugs remains urgent though hampered by a lack of knowledge regarding the molecular mechanisms of artemisinin resistance. Semisynthet
Mem. Inst. Oswaldo Cruz. Publicado em: 2015-04
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5. Rat vas deferens SERCA2 is modulated by Ca2+/calmodulin protein kinase II-mediated phosphorylation
Ca2+ pumps are important players in smooth muscle contraction. Nevertheless, little information is available about these pumps in the vas deferens. We have determined which subtype of sarco(endo)plasmic reticulum Ca2+-ATPase isoform (SERCA) is expressed in rat vas deferens (RVD) and its modulation by calmodulin (CaM)-dependent mechanisms. The thapsigargin-se
Braz J Med Biol Res. Publicado em: 19/03/2013
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6. Estudo do efeito relaxante de um novo composto doador de óxido nítrico em preparações de traquéias isoladas de ratos / Study of the relaxing effect of a new nitric oxide donor compound in preparations of isolated rat tracheae
This study evaluated the mechanisms of relaxation of two nitric oxide (NO) donors, ruthenium complex [Ru(terpy)(bdq)NO+]3+ (Terpy) and sodium nitroprusside (SNP) in isolated trachea of rats. The isolated trachea was sectioned into rings and contracted with carbachol in an organ chamber. The relaxing effect of Terpy and SNP was evaluated at increasing concent
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/02/2011
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7. Sildenafil decreases rat tracheal hyperresponsiveness to carbachol and changes canonical transient receptor potential gene expression after antigen challenge
Inhibition of type-5 phosphodiesterase by sildenafil decreases capacitative Ca2+ entry mediated by transient receptor potential proteins (TRPs) in the pulmonary artery. These families of channels, especially the canonical TRP (TRPC) subfamily, may be involved in the development of bronchial hyperresponsiveness, a hallmark of asthma. In the present study, we
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-06
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8. Lack of evidence for regulation of cardiac P-type ATPases and MAP kinases in transgenic mice with cardiac-specific overexpression of constitutively active α1B-adrenoceptors
The regulatory function of α1B-adrenoceptors in mammalian heart homeostasis is controversial. The objective of the present study was to characterize the expression/activity of key proteins implicated in cardiac calcium handling (Na+/K+-ATPase and Ca2+-ATPases) and growth (ERK1/2, JNK1/2 and p38) in mice with cardiac-selective overexpression of constitutivel
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-05
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9. Relaxamento do músculo liso traqueal de rato pelo eugenol. / Relaxation of rat tracheal smooth muscle by eugenol.
Eugenol, a phenol monoterpenoid, is the main component of clove oil. This study aims to evaluate the relaxation of tracheal smooth muscle of rat by eugenol and the mechanisms underlying these effects. We used preparations of trachea of male Wistar rats maintained in aerated modified Tyrode to record isometric muscle contractions. Increasing and cumulative co
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/02/2009
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10. Antimalarial drugs disrupt ion homeostasis in malarial parasites
Plasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling and also target for antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. chabaudi subcellular compartments. The AO could be released from the parasite or
Memórias do Instituto Oswaldo Cruz. Publicado em: 2007-06
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11. Solubilização e efeito de tapsigargina em Ca2+-ATPase de larva de Pachymerus nucleorum (Fabricius 1792) (Coleoptera: Chrysomelidae: Bruchinae)
CHAPTER I: Pachymerus nucleorum Fabricius 1792 is a beetle specie that predate babaçu coconut (Orbignya sp.). It belong to Bruchinae coleoptera subfamily, whose representants are know as seed bits. We found a precipitated fraction from larvae of P. nucleorum that is not solubilized by Triton X-100 (P3) and presents Ca2+-ATPase and K+/EDTA-ATPase activity. T
Publicado em: 2007
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12. Inhibition of the sarcoplasmic reticulum Ca2+ pump with thapsigargin to estimate the contribution of Na+-Ca2+ exchange to ventricular myocyte relaxation
Relaxation in the mammalian ventricle is initiated by Ca2+ removal from the cytosol, which is performed by three main transport systems: sarcoplasmic reticulum Ca2+-ATPase (SR-A), Na+-Ca2+ exchanger (NCX) and the so-called slow mechanisms (sarcolemmal Ca2+-ATPase and mitochondrial Ca2+ uptake). To estimate the relative contribution of each system to twitch r
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-12