Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

Abstract Peanut (Arachis hypogaea) is an important legume and oilseed crop, native to South America and grown in all tropical and temperate regions of the world. A simplified and rapid direct gene delivery system in peanut was developed by vortexing silicon carbide whiskers with callus and with plasmid harboring chitinase and hygromcin genes. The effects of callus age and whisker quantity on transformation efficiency were evaluated. Transformation efficiency (6.88%) was highest when 200 mg of whiskers were used with 5 µg plasmid for 2 g of 20-day-old callus. Hygromcin-resistant calli were reg...