Ultrasonic-extraction of phenolic compounds from Phyllanthus urinaria: optimization model and antioxidant activity

Abstract Ultrasonic-assisted extraction technique was used to extract phenolic compounds from Phyllanthus urinaria and response surface methodology was used to optimize the extraction parameters including ultrasonic time (X1), ethanol concentration (X2) and solid-liquid ratio (X3). A mathematical model of the tree parameters for the high extraction yield (Y) was established. Antioxidant activity of the phenolic compounds was investigated by DPPH free radicals, ABTS free radicals, hydroxyl free radicals and reducing power assay with vitamin C as a control. The results showed that the independen...