Timing of fluoride toothpaste use and enamel-dentin demineralization

It is well established that fluoride (F) prevents caries development by inhibiting demineralization and enhancing remineralization processes. However, it is not known which of these protective mechanisms is more important. In this double-blind, crossover in situ study conducted in three phases of 14 days each, 12 volunteers wore palatal appliances containing enamel and root dentin slabs, on which biofilm was allowed to accumulate under exposure to 20% sucrose solution 8×/day. F toothpaste was used once a day, either before the daily demineralizing episodes (in the morning) or after them (at n...