Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clus...