Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method

Abstract The objective of this study was to develop and validate a new HPLC method to quantify several flavonoids in the methanol extract prepared from the aerial parts of four Scutellaria L. taxa from flora of Turkey. A simple, sensitive and precise reversed phase HPLC-DAD method was developed and validated for simultaneous determination of six main flavonoids; scutellarein 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucuronopyranoside, apigenin 7-O-β-d-glucopyranoside, hispidulin 7-O-β-d-glucopyranoside, luteolin and apigenin. All standard compounds showed a good linearity (R 2 > 0.999)...