Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Trypsin inhibitors (TI) have crucial functions in plant defense against pests, and recent studies reveal their diversity in stress response. Disease and herbivore infestations are harmful to plant growth, but the response of TI in Cicer arietinum (L.) in Central India has been less studied. In the present study, we partially characterized the TI from Cicer arietinum (L.) seeds and evaluated its insecticidal potential against Helicoverpa armigera (Hübner). A 20 kDa trypsin inhibitor was purified to homogeneity by ammonium sulfate precipitation and chromatographies with Sephadex G-100 and dieth...

Texto completo