Propagación in vitro de Bucquetia glutinosa, especie endémica de los Paramos colombianos

Abstract In vitro multiplication protocol of B. glutinosa was established by seeds collected in the Paramo of Rabanal (Boyacá-Colombia), this species is useful in programs for the conservation and restoration of moorland ecosystems. During the establishment of in vitro cultures, 57% of aseptic seeds were obtained with 5 and 10% NaOCl, after 30 days of culture in MS-1, 61.4% of them developing normal appearance seedlings; 75% of the micro-stems cultivated in MS / 2 + 0.5 mg l-1 IBA reached an average length of 2.06 cm, formed up to 10 axillary buds and among these, around two and four basal sh...