Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants

Abstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemented with 1.34 μM NAA and 2.27 μM TDZ resulted in better response on PBLs, and subsequent shoot differentiation (55.25 shoots per explant), and in better rooting number and root length...