Isolation and molecular characterization of Campylobacter jejuni from chicken and human stool samples in Egypt

Abstract Two hundred broiler chicken samples, 160 laying chicken samples and 75 human stool samples were collected in Egypt. The samples were microbiologically examined, and the C. jejuni isolates were confirmed biochemically and by PCR targeting the mapA gene of C. jejuni. The prevalence of the cadF virulence gene was then determined using PCR. A total of 17.33%, 17% and 11.87% of human stool, broiler chicken and laying chicken samples, respectively, were positive for C. jejuni, with a total of 66 Campylobacter jejuni isolates (15.17%). Ten C. jejuni isolates (15.15%) carried the cadF virulen...