INFLUENCE OF HYDRAULIC RETENTION TIME ON HYDROGEN PRODUCTION BY TREATING CHEESE WHEY WASTEWATER IN ANAEROBIC FLUIDIZED BED BIOREACTOR - AN APPROACH FOR DEVELOPING COUNTRIES

Abstract This study assessed the effect of hydraulic retention time (HRT) on biohydrogen production by dark fermentation using an anaerobic fluidized bed reactor fed with cheese whey synthetic wastewater. The reactor of 1.2 L was operated with an approximate carbohydrate concentration of 2800 mg L-1, and HRTs of 6, 4, 2, 1 and 0.5 h. Acetic and butyric acids were the main metabolites produced in the reactor. The hydrogen production increased from 0.039 to 1.43 L-H2 h-1 L-1-reactor as the HRT decreased from 6 to 0.5 h. The best hydrogen yield (HY) (2.73 mol-H2 mol-carbohydrate-1) and carbohydra...